Induction of transferrin secretion in murine sertoli cells by FSH and IL-1: The possibility of different mechanism(s) of regulation

Mahmoud Huleihel, Daniel Zeyse, Mahmoud Huleihel, Daniel Zeyse, Eitan Lunenfeld, Melanie Zeyse, Moshe Mazor, Mahmoud Huleihel, Daniel Zeyse, Eitan Lunenfeld, Moshe Mazor, Daniel Zeyse, Melanie Zeyse

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    19 Scopus citations

    Abstract

    In the present study we examined the capacity of interleukin-1 (IL-1) α, β, interleukin-1 receptor antagonist (IL-1ra) and follicle stimulating hormone (FSH) to induce transferrin secretion by Sertoli cells under in vitro conditions. Primary Sertoli cell (SC) cultures from immature mice secreted constitutively transferrin. Stimulation of these cultures with IL-1α, IL-1β significantly increased their capacity to secrete transferrin. Addition of IL-1ra to unstimulated SC cultures did not affect their capacity to secrete transferrin. Stimulation of SC cultures with a combination of both IL-1α and FSH or IL-1β and FSH showed additive effect between IL-1 and FSH in their capacity to induce transferrin secretion by these cells. However, stimulation of Sertoli cells with a combination of both IL-1ra and FSH did not affect their capacity to secrete transferrin compared with FSH-stimulated cultures. Our results may suggest the involvement of testicular paracrine/autocrine factors (IL-1) and endocrine (FSH) factors in the regulation of transferrin secretion by SC. This capacity seems to be differently regulated by these factors. Thus, IL-1α and β may directly affect physiological functions of the testis; which may suggest their involvement in the regulation of spermatogenesis and spermiogenesis processes and male fertility.

    Original languageEnglish
    Pages (from-to)112-117
    Number of pages6
    JournalAmerican Journal of Reproductive Immunology
    Volume47
    Issue number2
    DOIs
    StatePublished - 4 Jun 2002

    Keywords

    • Follicle stimulating hormone
    • Interleukin-1
    • Interleukin-1 receptor antagonist
    • Male fertility
    • Sertoli cells
    • Testis

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