Inferring primase-DNA specific recognition using a data driven approach

Adam Soffer, Sarah A. Eisdorfer, Morya Ifrach, Stefan Ilic, Ariel Afek, Hallel Schussheim, Dan Vilenchik, Barak Akabayov

Research output: Contribution to journalArticlepeer-review

Abstract

DNA-protein interactions play essential roles in all living cells. Understanding of how features embedded in the DNA sequence affect specific interactions with proteins is both challenging and important, since it may contribute to finding the means to regulate metabolic pathways involving DNA-protein interactions. Using a massive experimental benchmark dataset of binding scores for DNA sequences and a machine learning workflow, we describe the binding to DNA of T7 primase, as a model system for specific DNA-protein interactions. Effective binding of T7 primase to its specific DNA recognition sequences triggers the formation of RNA primers that serve as Okazaki fragment start sites during DNA replication.

Original languageEnglish
Pages (from-to)11447-11458
Number of pages12
JournalNucleic Acids Research
Volume49
Issue number20
DOIs
StatePublished - 18 Nov 2021

ASJC Scopus subject areas

  • Genetics

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