Inflorescence bud protease(s) against dehydrin-like inflorescence bud proteins of Pistacia vera

Irena Peri, Radoslava Matusova, Patricia Smirnoff, Yehudith Birk, Avi Golan-Goldhirsh

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1 Scopus citations

Abstract

This paper reports on a proteolytic activity extracted from Pistacia vera L. (pistachio) inflorescence buds, found in male and female tissues. The proteolytic activity reaches a peak at the beginning of bud opening and flowering and is directed against inflorescence bud dehydrin-like proteins of pistachio, IBP32 and IBP27. The effect of protease inhibitors indicated that the protease(s) belong to a serine-protease-like family and are not cysteine, acid or metallo-proteases. The proteolytic activity was strongly inhibited by Nα-p-tosyl-L-lysine chloromethyl ketone and to a lesser extent by N-tosyl-L-phenylalanine chloromethyl ketone, suggesting mainly trypsin-like specificity or broader serine-protease specificity. It is suggested that the proteolytic activity is important in the mobilization of nitrogen reserves stored in the bud storage proteins during dormancy to support the fast-developing inflorescence in spring after bud dormancy break.

Original languageEnglish
Pages (from-to)51-56
Number of pages6
JournalPlant Physiology and Biochemistry
Volume37
Issue number1
DOIs
StatePublished - 1 Jan 1999

Keywords

  • Deciduous
  • Dioecious
  • Dormancy
  • Inflorescence bud proteins
  • Proteolytic activity
  • Serine-protease

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