Influence of perfluorinated carboxylic acids on ion-pair reversed-phase high-performance liquid chromatographic separation of betacyanins and 17-decarboxy-betacyanins

Sławomir Wybraniec, Yosef Mizrahi

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

The ability of trifluoroacetic acid, pentafluoropropionic acid and heptafluorobutyric acid to act as ion-pairing agents for betacyanins and 17-decarboxy-betacyanins during HPLC analysis on a Luna C18(2) reversed-phase column is reported. While the perfluorinated carboxylic acids affect the retention of both groups of compounds by changing the pH of the mobile phase, the possibility of ion-pair chromatography for 17-decarboxy-betacyanins was noticed. In order to explain the accessibility of the positive charge for the counter-anion in decarboxy-betacyanins, the mesomeric structures of the polymethine system at low pH (around a value of 1.5), when the carboxylic group in the 2 position is protonated, should be taken into consideration.

Original languageEnglish
Pages (from-to)97-101
Number of pages5
JournalJournal of Chromatography A
Volume1029
Issue number1-2
DOIs
StatePublished - 12 Mar 2004

Keywords

  • Betacyanins
  • Betalains
  • Betanin
  • Carboxylic acids
  • Decarboxy-betacyanins
  • Fluorinated carboxylic acids
  • Hylocerenin
  • Hylocereus polyrhizus
  • Phyllocactin

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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