Abstract
The ability of trifluoroacetic acid, pentafluoropropionic acid and heptafluorobutyric acid to act as ion-pairing agents for betacyanins and 17-decarboxy-betacyanins during HPLC analysis on a Luna C18(2) reversed-phase column is reported. While the perfluorinated carboxylic acids affect the retention of both groups of compounds by changing the pH of the mobile phase, the possibility of ion-pair chromatography for 17-decarboxy-betacyanins was noticed. In order to explain the accessibility of the positive charge for the counter-anion in decarboxy-betacyanins, the mesomeric structures of the polymethine system at low pH (around a value of 1.5), when the carboxylic group in the 2 position is protonated, should be taken into consideration.
| Original language | English |
|---|---|
| Pages (from-to) | 97-101 |
| Number of pages | 5 |
| Journal | Journal of Chromatography A |
| Volume | 1029 |
| Issue number | 1-2 |
| DOIs | |
| State | Published - 12 Mar 2004 |
Keywords
- Betacyanins
- Betalains
- Betanin
- Carboxylic acids
- Decarboxy-betacyanins
- Fluorinated carboxylic acids
- Hylocerenin
- Hylocereus polyrhizus
- Phyllocactin
ASJC Scopus subject areas
- Analytical Chemistry
- Biochemistry
- Organic Chemistry
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