Information from combined 1H and 31P NMR studies of cell extracts: Differences in metabolism between drug-sensitive and drug-resistant MCF-7 human breast cancer cells

Ofer Kaplan; Peter C.M. van Zijl; Jack S. Cohen

doi:10.1016/0006-291X(90)90343-L

Information from combined ¹H and ³¹P NMR studies of cell extracts: Differences in metabolism between drug-sensitive and drug-resistant MCF-7 human breast cancer cells

Ofer Kaplan, Peter C.M. van Zijl, Jack S. Cohen

Research output: Contribution to journal › Article › peer-review

42 Scopus citations

Abstract

Combined analysis of both ¹H and ³¹P NMR spectra of extracts of drug-sensitive and multidrug-resistant MCF-7 human breast cancer cells enabled quantitative comparisons between the concentrations of major metabolites, as well as of their precursors. In resistant cells high energy phosphorus compounds, phosphocreatine and ATP, and also their precursors, creatine and ADP, were elevated compared to the sensitive cells. In phospholipid metabolism, the sensitive cells showed higher phosphocholine and phosphoethanolamine concentrations than the resistant cells, but choline levels were similar. These results delineated the differences in control of metabolic pathways between drug-sensitive and drug-resistant cells.

Original language	English
Pages (from-to)	383-390
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	169
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(90)90343-L
State	Published - 15 Jun 1990
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1016/0006-291X(90)90343-L

Cite this

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title = "Information from combined 1H and 31P NMR studies of cell extracts: Differences in metabolism between drug-sensitive and drug-resistant MCF-7 human breast cancer cells",

abstract = "Combined analysis of both 1H and 31P NMR spectra of extracts of drug-sensitive and multidrug-resistant MCF-7 human breast cancer cells enabled quantitative comparisons between the concentrations of major metabolites, as well as of their precursors. In resistant cells high energy phosphorus compounds, phosphocreatine and ATP, and also their precursors, creatine and ADP, were elevated compared to the sensitive cells. In phospholipid metabolism, the sensitive cells showed higher phosphocholine and phosphoethanolamine concentrations than the resistant cells, but choline levels were similar. These results delineated the differences in control of metabolic pathways between drug-sensitive and drug-resistant cells.",

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Information from combined ¹H and ³¹P NMR studies of cell extracts: Differences in metabolism between drug-sensitive and drug-resistant MCF-7 human breast cancer cells. / Kaplan, Ofer; van Zijl, Peter C.M.; Cohen, Jack S.
In: Biochemical and Biophysical Research Communications, Vol. 169, No. 2, 15.06.1990, p. 383-390.

Research output: Contribution to journal › Article › peer-review

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AU - Cohen, Jack S.

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