Infrared surface plasmon spectroscopy decodes early processes in epithelial host cells upon enteropathogenic Escherichia coli infection

Victor Yashunsky, Benjamin Aroeti

Research output: Chapter in Book/Report/Conference proceedingChapterpeer-review

Abstract

Enteropathogenic Escherichia coli (EPEC) is a generally noninvasive bacterial pathogen that causes diarrhea in humans. This microbe infects mainly the enterocytes of the small intestine. In this chapter we describe newly developed method, infrared surface plasmon resonance (IR-SPR) spectroscopy, for sensing pathogen infection of living cells. The IR-SPR method enables real-time and label-free monitoring of EPEC infection through highly sensitive measurement of the refractive index and height of the host epithelial cell monolayer. Our findings indicate the great potential of the IR-SPR tool to study the dynamics of host-pathogen interactions with high spatiotemporal sensitivity.

Original languageEnglish
Title of host publicationLabel-Free Biosensor Methods in Drug Discovery
EditorsYe Fang
PublisherSpringer New York
Pages353-371
Number of pages19
Volume53
ISBN (Electronic)9781493926176
ISBN (Print)9781493926169
DOIs
StatePublished - 6 Apr 2015
Externally publishedYes

Publication series

NameMethods in Pharmacology and Toxicology
PublisherHumana Press
ISSN (Print)1557-2153

Keywords

  • EPEC
  • Epithelial host cells
  • Infrared
  • Spectroscopy
  • Surface plasmon resonance

ASJC Scopus subject areas

  • General Medicine
  • Pharmacology, Toxicology and Pharmaceutics (all)

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