Inhibition of restriction endonucleases by biofunctionalized silver nanoparticles: An in vitro study

Hemant P. Borase; Chandrashekhar D. Patil; Rahul B. Salunkhe; Rahul K. Suryawanshi; Bipinchandra K. Salunke; Satish V. Patil

doi:10.1016/j.matlet.2014.07.055

Inhibition of restriction endonucleases by biofunctionalized silver nanoparticles: An in vitro study

Hemant P. Borase, Chandrashekhar D. Patil, Rahul B. Salunkhe, Rahul K. Suryawanshi, Bipinchandra K. Salunke, Satish V. Patil

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Ecofriendly synthesis of metal nanoparticles and their unique properties hold promise for innovative therapeutic applications. In the present study, rapid, novel, low cost method for synthesis of silver nanoparticles (AgNPs) using aqueous leaves extract of medicinal plant Euphorbia heterophylla (E. heterophylla) is reported. AgNPs synthesized by plant extract exhibited absorption peak at 422 nm in UV-vis spectroscopy. Transmission electron microscopy micrographs revealed presence of AgNPs with average size of 13 nm with grain and triangular shapes. Fourier Transform Infrared Spectroscopy analysis revealed role of leaves metabolites as reducing and capping agent in synthesis of AgNPs. X ray diffraction pattern and selected area electron diffraction study confirmed crystalline nature of AgNPs. Inhibition of DNA cutting activity of restriction endonucleases (EcoRI, HindIII and BamHI) by biofunctionalized AgNPs in agarose gel electrophoresis is first time reported in the present study. This potential of AgNPs can be useful for enhancing effectiveness of phage therapy by acting as synergistic cocktail with bacteriophages to kill bacteria via inhibition of their restriction endonucleases.

Original language	English
Pages (from-to)	24-26
Number of pages	3
Journal	Materials Letters
Volume	134
DOIs	https://doi.org/10.1016/j.matlet.2014.07.055
State	Published - 1 Nov 2014
Externally published	Yes

Keywords

Biosynthesis
Phage therapy
Restriction endonucleases inhibition
Silver nanoparticles

ASJC Scopus subject areas

General Materials Science
Condensed Matter Physics
Mechanics of Materials
Mechanical Engineering

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10.1016/j.matlet.2014.07.055

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title = "Inhibition of restriction endonucleases by biofunctionalized silver nanoparticles: An in vitro study",

abstract = "Ecofriendly synthesis of metal nanoparticles and their unique properties hold promise for innovative therapeutic applications. In the present study, rapid, novel, low cost method for synthesis of silver nanoparticles (AgNPs) using aqueous leaves extract of medicinal plant Euphorbia heterophylla (E. heterophylla) is reported. AgNPs synthesized by plant extract exhibited absorption peak at 422 nm in UV-vis spectroscopy. Transmission electron microscopy micrographs revealed presence of AgNPs with average size of 13 nm with grain and triangular shapes. Fourier Transform Infrared Spectroscopy analysis revealed role of leaves metabolites as reducing and capping agent in synthesis of AgNPs. X ray diffraction pattern and selected area electron diffraction study confirmed crystalline nature of AgNPs. Inhibition of DNA cutting activity of restriction endonucleases (EcoRI, HindIII and BamHI) by biofunctionalized AgNPs in agarose gel electrophoresis is first time reported in the present study. This potential of AgNPs can be useful for enhancing effectiveness of phage therapy by acting as synergistic cocktail with bacteriophages to kill bacteria via inhibition of their restriction endonucleases.",

keywords = "Biosynthesis, Phage therapy, Restriction endonucleases inhibition, Silver nanoparticles",

author = "Borase, {Hemant P.} and Patil, {Chandrashekhar D.} and Salunkhe, {Rahul B.} and Suryawanshi, {Rahul K.} and Salunke, {Bipinchandra K.} and Patil, {Satish V.}",

note = "Funding Information: Hemant P. Borase is thankful to Department of Science and Technology, Government of India for awarding a DST-INSPIRE fellowship (Grant File no. DST/INSPIRE Fellowship/2011[149] ). ",

year = "2014",

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day = "1",

doi = "10.1016/j.matlet.2014.07.055",

language = "English",

volume = "134",

pages = "24--26",

journal = "Materials Letters",

issn = "0167-577X",

publisher = "Elsevier B.V.",

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T1 - Inhibition of restriction endonucleases by biofunctionalized silver nanoparticles

T2 - An in vitro study

AU - Borase, Hemant P.

AU - Patil, Chandrashekhar D.

AU - Salunkhe, Rahul B.

AU - Suryawanshi, Rahul K.

AU - Salunke, Bipinchandra K.

AU - Patil, Satish V.

N1 - Funding Information: Hemant P. Borase is thankful to Department of Science and Technology, Government of India for awarding a DST-INSPIRE fellowship (Grant File no. DST/INSPIRE Fellowship/2011[149] ).

PY - 2014/11/1

Y1 - 2014/11/1

N2 - Ecofriendly synthesis of metal nanoparticles and their unique properties hold promise for innovative therapeutic applications. In the present study, rapid, novel, low cost method for synthesis of silver nanoparticles (AgNPs) using aqueous leaves extract of medicinal plant Euphorbia heterophylla (E. heterophylla) is reported. AgNPs synthesized by plant extract exhibited absorption peak at 422 nm in UV-vis spectroscopy. Transmission electron microscopy micrographs revealed presence of AgNPs with average size of 13 nm with grain and triangular shapes. Fourier Transform Infrared Spectroscopy analysis revealed role of leaves metabolites as reducing and capping agent in synthesis of AgNPs. X ray diffraction pattern and selected area electron diffraction study confirmed crystalline nature of AgNPs. Inhibition of DNA cutting activity of restriction endonucleases (EcoRI, HindIII and BamHI) by biofunctionalized AgNPs in agarose gel electrophoresis is first time reported in the present study. This potential of AgNPs can be useful for enhancing effectiveness of phage therapy by acting as synergistic cocktail with bacteriophages to kill bacteria via inhibition of their restriction endonucleases.

AB - Ecofriendly synthesis of metal nanoparticles and their unique properties hold promise for innovative therapeutic applications. In the present study, rapid, novel, low cost method for synthesis of silver nanoparticles (AgNPs) using aqueous leaves extract of medicinal plant Euphorbia heterophylla (E. heterophylla) is reported. AgNPs synthesized by plant extract exhibited absorption peak at 422 nm in UV-vis spectroscopy. Transmission electron microscopy micrographs revealed presence of AgNPs with average size of 13 nm with grain and triangular shapes. Fourier Transform Infrared Spectroscopy analysis revealed role of leaves metabolites as reducing and capping agent in synthesis of AgNPs. X ray diffraction pattern and selected area electron diffraction study confirmed crystalline nature of AgNPs. Inhibition of DNA cutting activity of restriction endonucleases (EcoRI, HindIII and BamHI) by biofunctionalized AgNPs in agarose gel electrophoresis is first time reported in the present study. This potential of AgNPs can be useful for enhancing effectiveness of phage therapy by acting as synergistic cocktail with bacteriophages to kill bacteria via inhibition of their restriction endonucleases.

KW - Biosynthesis

KW - Phage therapy

KW - Restriction endonucleases inhibition

KW - Silver nanoparticles

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Inhibition of restriction endonucleases by biofunctionalized silver nanoparticles: An in vitro study

Abstract

Keywords

ASJC Scopus subject areas

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