Inhibition of Staphylococcus aureus biofilm-forming functional amyloid by molecular tweezers

Ravit Malishev, Nir Salinas, James Gibson, Angela Bailey Eden, Joel Mieres-Perez, Yasser B. Ruiz-Blanco, Orit Malka, Sofiya Kolusheva, Frank Gerrit Klärner, Thomas Schrader, Elsa Sanchez-Garcia, Chunyu Wang, Meytal Landau, Gal Bitan, Raz Jelinek

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Biofilms are rigid and largely impenetrable three-dimensional matrices constituting virulence determinants of various pathogenic bacteria. Here, we demonstrate that molecular tweezers, unique supramolecular artificial receptors, modulate biofilm formation of Staphylococcus aureus. In particular, the tweezers affect the structural and assembly properties of phenol-soluble modulin α1 (PSMα1), a biofilm-scaffolding functional amyloid peptide secreted by S. aureus. The data reveal that CLR01, a diphosphate tweezer, exhibits significant S. aureus biofilm inhibition and disrupts PSMα1 self-assembly and fibrillation, likely through inclusion of lysine side chains of the peptide. In comparison, different peptide binding occurs in the case of CLR05, a tweezer containing methylenecarboxylate units, which exhibits lower affinity for the lysine residues yet disrupts S. aureus biofilm more strongly than CLR01. Our study points to a possible role for molecular tweezers as potent biofilm inhibitors and antibacterial agents, particularly against untreatable biofilm-forming and PSM-producing bacteria, such as methicillin-resistant S. aureus.

Original languageEnglish
Pages (from-to)1310-1320.e5
JournalCell Chemical Biology
Volume28
Issue number9
DOIs
StatePublished - 16 Sep 2021

Keywords

  • MRSA
  • PSMa1
  • Staphylococcus aureus
  • amyloid peptides
  • antibacterial
  • biofilm
  • functional amyloid
  • molecular tweezer
  • phenol-soluble modulins

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

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