Inhibition of Tomato Root Growth in Culture by a Verticillium Dahliae Phytotoxin

Janeta Orenstein, A. Nachmias, H. Lips

    Research output: Contribution to journalArticlepeer-review

    3 Scopus citations

    Abstract

    To assess the relationship between Verticilliun dahliae toxin and the stunting effect in V. dahliae-infected plants, an assay was developed based on the inhibition of elongation of cultured tomato roots. The toxin inhibited growth of roots and development of secondary roots in disease-susceptible plants, tomato cv. 'Hosen-Eilon'(veve) and eggplant, whereas root growth was enhanced in tomato cv. 'VF-134', which carries the Ve gene for resistance, and in a non-host plant, onion. The toxin also inhibited the incorporation of [14C]-glutamic acid and [14C]-uridine into TCA-precipitable material in susceptible but not in resistant tomato roots. Cells from toxin-treated susceptible roots were smaller than those from resistant roots, suggesting that the toxin impeded cell growth rather than cell division. Since toxin-induced stunting cannot be detected using leaf bioassays, we suggest that the root inhibition assay can be used as a reliable tool for Verticillium disease tolerance screening studies with the V. dahliae toxin as probe.

    Original languageEnglish
    Pages (from-to)269-278
    Number of pages10
    JournalPhytoparasitica
    Volume17
    Issue number4
    DOIs
    StatePublished - 1 Dec 1989

    Keywords

    • Verticillium wilt
    • root bioassay
    • stunting

    ASJC Scopus subject areas

    • Plant Science
    • Insect Science

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