TY - JOUR
T1 - Injectable Cardiac Cell Microdroplets for Tissue Regeneration
AU - Gal, Idan
AU - Edri, Reuven
AU - Noor, Nadav
AU - Rotenberg, Matan
AU - Namestnikov, Michael
AU - Cabilly, Itai
AU - Shapira, Assaf
AU - Dvir, Tal
N1 - Publisher Copyright:
© 2020 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2020/2/1
Y1 - 2020/2/1
N2 - One of the strategies for heart regeneration includes cell delivery to the defected heart. However, most of the injected cells do not form quick cell–cell or cell–matrix interactions, therefore, their ability to engraft at the desired site and improve heart function is poor. Here, the use of a microfluidic system is reported for generating personalized hydrogel-based cellular microdroplets for cardiac cell delivery. To evaluate the system's limitations, a mathematical model of oxygen diffusion and consumption within the droplet is developed. Following, the microfluidic system's parameters are optimized and cardiac cells from neonatal rats or induced pluripotent stem cells are encapsulated. The morphology and cardiac specific markers are assessed and cell function within the droplets is analyzed. Finally, the cellular droplets are injected to mouse gastrocnemius muscle to validate cell retention, survival, and maturation within the host tissue. These results demonstrate the potential of this approach to generate personalized cellular microtissues, which can be injected to distinct regions in the body for treating damaged tissues.
AB - One of the strategies for heart regeneration includes cell delivery to the defected heart. However, most of the injected cells do not form quick cell–cell or cell–matrix interactions, therefore, their ability to engraft at the desired site and improve heart function is poor. Here, the use of a microfluidic system is reported for generating personalized hydrogel-based cellular microdroplets for cardiac cell delivery. To evaluate the system's limitations, a mathematical model of oxygen diffusion and consumption within the droplet is developed. Following, the microfluidic system's parameters are optimized and cardiac cells from neonatal rats or induced pluripotent stem cells are encapsulated. The morphology and cardiac specific markers are assessed and cell function within the droplets is analyzed. Finally, the cellular droplets are injected to mouse gastrocnemius muscle to validate cell retention, survival, and maturation within the host tissue. These results demonstrate the potential of this approach to generate personalized cellular microtissues, which can be injected to distinct regions in the body for treating damaged tissues.
KW - cell delivery
KW - droplets
KW - hydrogels
KW - microfluidics
KW - microscale
UR - http://www.scopus.com/inward/record.url?scp=85078854744&partnerID=8YFLogxK
U2 - 10.1002/smll.201904806
DO - 10.1002/smll.201904806
M3 - Article
C2 - 32003928
AN - SCOPUS:85078854744
SN - 1613-6810
VL - 16
JO - Small
JF - Small
IS - 8
M1 - 1904806
ER -