Innovative approach for urease inhibition by Ficus carica extract-fabricated silver nanoparticles: An in vitro study

Hemant P. Borase, Rahul B. Salunkhe, Chandrashekhar D. Patil, Rahul K. Suryawanshi, Bipinchandra K. Salunke, Nilesh D. Wagh, Satish V. Patil

Research output: Contribution to journalArticlepeer-review

22 Scopus citations

Abstract

In the present study, a rapid, low-cost, and ecofriendly method of stable silver nanoparticles (AgNPs) synthesis using leaves extract of Ficus carica (F. carica), a plant with diverse metabolic consortium, is reported for the first time. An absorption peak at 422 nm in UV-Vis spectroscopy, a spherical shape with an average size of 21 nm in transmission electron microscopy, and crystalline nature in X-ray powder diffraction studies were observed for the synthesized AgNPs. Fourier transform infrared analysis indicated that proteins of F. carica might have a vital role in AgNP synthesis and stabilization. AgNPs were found to inhibit urease, a key enzyme responsible for the survival and pathogenesis of the bacterium, Helicobacter pylori. Inhibition of urease by AgNPs was monitored spectrophotometrically by the evaluation of ammonia release. The urease inhibition potential of AgNPs can be explored in the treatment of H. pylori by preparing novel combinations of standard drugs with AgNPs- or AgNPs-encapsulated drug molecules.

Original languageEnglish
Pages (from-to)780-784
Number of pages5
JournalBiotechnology and Applied Biochemistry
Volume62
Issue number6
DOIs
StatePublished - 1 Nov 2015
Externally publishedYes

Keywords

  • FT-IR
  • Helicobacter pylori
  • biomaterials
  • silver nanoparticles
  • urease inhibitors

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Molecular Medicine
  • Biomedical Engineering
  • Applied Microbiology and Biotechnology
  • Drug Discovery
  • Process Chemistry and Technology

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