Insertion mutagenesis of embryonal carcinoma cells by retroviruses

Walter King; Mayuri D. Patel; Leslie I. Lobel; Stephen P. Goff; M. Chi Nguyen-Huu

doi:10.1126/science.3838595

Insertion mutagenesis of embryonal carcinoma cells by retroviruses

Walter King, Mayuri D. Patel, Leslie I. Lobel, Stephen P. Goff, M. Chi Nguyen-Huu

Research output: Contribution to journal › Article › peer-review

88 Scopus citations

Abstract

Mutagenesis was studied in cultured F9 embryonal carcinoma cells infected with a variant of Moloney murine leukemia virus. Proviral insertion induced the inactivation of the hypoxanthine phosphoribosyltransferase locus, and the virus was used to isolate the mutated genes rapidly. Mutagenesis by these methods may be useful for the genetic dissection of the various mammalian cell phenotypes.

Original language	English
Pages (from-to)	554-558
Number of pages	5
Journal	Science
Volume	228
Issue number	4699
DOIs	https://doi.org/10.1126/science.3838595
State	Published - 1 Jan 1985
Externally published	Yes

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title = "Insertion mutagenesis of embryonal carcinoma cells by retroviruses",

abstract = "Mutagenesis was studied in cultured F9 embryonal carcinoma cells infected with a variant of Moloney murine leukemia virus. Proviral insertion induced the inactivation of the hypoxanthine phosphoribosyltransferase locus, and the virus was used to isolate the mutated genes rapidly. Mutagenesis by these methods may be useful for the genetic dissection of the various mammalian cell phenotypes.",

author = "Walter King and Patel, {Mayuri D.} and Lobel, {Leslie I.} and Goff, {Stephen P.} and Nguyen-Huu, {M. Chi}",

year = "1985",

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doi = "10.1126/science.3838595",

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AU - King, Walter

AU - Patel, Mayuri D.

AU - Lobel, Leslie I.

AU - Goff, Stephen P.

AU - Nguyen-Huu, M. Chi

PY - 1985/1/1

Y1 - 1985/1/1

N2 - Mutagenesis was studied in cultured F9 embryonal carcinoma cells infected with a variant of Moloney murine leukemia virus. Proviral insertion induced the inactivation of the hypoxanthine phosphoribosyltransferase locus, and the virus was used to isolate the mutated genes rapidly. Mutagenesis by these methods may be useful for the genetic dissection of the various mammalian cell phenotypes.

AB - Mutagenesis was studied in cultured F9 embryonal carcinoma cells infected with a variant of Moloney murine leukemia virus. Proviral insertion induced the inactivation of the hypoxanthine phosphoribosyltransferase locus, and the virus was used to isolate the mutated genes rapidly. Mutagenesis by these methods may be useful for the genetic dissection of the various mammalian cell phenotypes.

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U2 - 10.1126/science.3838595

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VL - 228

SP - 554

EP - 558

JO - Science

JF - Science

IS - 4699

ER -

Insertion mutagenesis of embryonal carcinoma cells by retroviruses

Abstract

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