Insertion mutagenesis of embryonal carcinoma cells by retroviruses

Walter King, Mayuri D. Patel, Leslie I. Lobel, Stephen P. Goff, M. Chi Nguyen-Huu

Research output: Contribution to journalArticlepeer-review

88 Scopus citations


Mutagenesis was studied in cultured F9 embryonal carcinoma cells infected with a variant of Moloney murine leukemia virus. Proviral insertion induced the inactivation of the hypoxanthine phosphoribosyltransferase locus, and the virus was used to isolate the mutated genes rapidly. Mutagenesis by these methods may be useful for the genetic dissection of the various mammalian cell phenotypes.

Original languageEnglish
Pages (from-to)554-558
Number of pages5
Issue number4699
StatePublished - 1 Jan 1985
Externally publishedYes

ASJC Scopus subject areas

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