Insights into enhanced thermostability of a cellulosomal enzyme

Johanna Stern, Michael Anbar, Sarah Moraïs, Raphael Lamed, Edward A. Bayer

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Improved stability of cellulosomal enzymes is of great significance in order to provide efficient degradation of cellulosic derivatives for production of biofuels. In previous reports, we created a quadruple mutant of the endoglucanase Cel8A from Clostridium thermocellum resulting from a combination of both random error-prone PCR and a bioinformatics-based consensus mutagenesis approach. The quadruple mutant exhibited an increased half-life of activity by 14-fold at 85 °C with no apparent loss of catalytic activity compared to the wild-type form. Connection of the wild-type enzyme to its respective cohesin partner conferred increased thermostability, but no increase was observed for the cohesin-complexed mutant enzyme. The mutant and the wild-type enzymes were integrated into divalent chimeric scaffoldins with a family 48 exoglucanase partner, and the cellulose-degradation activities of resultant designer cellulosomes were examined. Despite the heightened thermostability of the mutant as a free enzyme, its substitution for the wild-type endoglucanase within the cellulosome context failed to exhibit an improvement in overall degradation of cellulose.

Original languageEnglish
Pages (from-to)78-84
Number of pages7
JournalCarbohydrate Research
Volume389
Issue number1
DOIs
StatePublished - 7 May 2014
Externally publishedYes

Keywords

  • Cellulosome
  • Cohesin
  • Dockerin
  • Endoglucanase
  • Exoglucanase
  • Thermostability

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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