Integrating Rio1 activities discloses its nutrient-activated network in Saccharomyces cerevisiae

Maria G. Iacovella, Michael Bremang, Omer Basha, Luciano Giacò, Walter Carotenuto, Cristina Golfieri, Barnabas Szakal, Marianna Dal Maschio, Valentina Infantino, Galina V. Beznoussenko, Chinnu R. Joseph, Clara Visintin, Alexander A. Mironov, Rosella Visintin, Dana Branzei, Sébastien Ferreira-Cerca, Esti Yeger-Lotem, Peter De Wulf

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


The Saccharomyces cerevisiae kinase/adenosine triphosphatase Rio1 regulates rDNA transcription and segregation, pre-rRNA processing and small ribosomal subunit maturation. Other roles are unknown. When overexpressed, human ortholog RIOK1 drives tumor growth and metastasis. Likewise, RIOK1 promotes 40S ribosomal subunit biogenesis and has not been characterized globally. We show that Rio1 manages directly and via a series of regulators, an essential signaling network at the protein, chromatin and RNA levels. Rio1 orchestrates growth and division depending on resource availability, in parallel to the nutrient-activated Tor1 kinase. To define the Rio1 network, we identified its physical interactors, profiled its target genes/transcripts, mapped its chromatin-binding sites and integrated our data with yeast's protein-protein and protein-DNA interaction catalogs using network computation. We experimentally confirmed network components and localized Rio1 also to mitochondria and vacuoles. Via its network, Rio1 commands protein synthesis (ribosomal gene expression, assembly and activity) and turnover (26S proteasome expression), and impinges on metabolic, energy-production and cell-cycle programs. We find that Rio1 activity is conserved to humans and propose that pathological RIOK1 may fuel promiscuous transcription, ribosome production, chromosomal instability, unrestrained metabolism and proliferation; established contributors to cancer. Our study will advance the understanding of numerous processes, here revealed to depend on Rio1 activity.

Original languageEnglish
Pages (from-to)7586-7611
Number of pages26
JournalNucleic Acids Research
Issue number15
StatePublished - 6 Sep 2018

ASJC Scopus subject areas

  • Genetics


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