Integrative analysis of transcriptional profile reveals LINC00052 as a suppressor of breast cancer cell migration

Jose Manuel Sanchez-Lopez, Edna Ayerim Mandujano-Tinoco, Alfredo Garcia-Venzor, Laura Fatima Lozada-Rodriguez, Cecilia Zampedri, Salvador Uribe-Carvajal, Jorge Melendez-Zajgla, Vilma Maldonado, Floria Lizarraga

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

BACKGROUND: Long-non-coding RNAs, a class of transcripts with lengths > 200 nt, play key roles in tumour progression. Previous reports revealed that LINC00052 (long intergenic non-coding RNA 00052) was strongly downregulated during breast cancer multicellular spheroids formation and suggested a role in cell migration and oxidative metabolism. OBJECTIVE: To examine the function of LINC00052 in MCF-7 breast cancer cells. METHODS: Loss-of-function studies were performed to evaluate LINC00052 role on MCF-7 breast cancer cells. Microarray expression assays were performed to determine genes and cellular functions modified after LINC00052 knockdown. Next, the impact of LINC00052 depletion on MCF-7 cell respiration and migration was evaluated. RESULTS: 1,081 genes were differentially expressed upon LINC00052 inhibition. Gene set enrichment analysis, Gene Ontology and Key Pathway Advisor analysis showed that signalling networks related to cell migration and oxidative phosphorylation were enriched. However, whereas LINC00052 knockdown in MCF-7 cells revealed marginal difference in oxygen consumption rates when compared with control cells, LINC00052 inhibition enhanced cell migration in vitro and in vivo, as observed using a Zebrafish embryo xenotransplant model. CONCLUSION: Our data show that LINC00052 modulates MCF-7 cell migration. Genome-wide microarray experiments suggest that cancer cell migration is affected by LINC00052 through cytoskeleton modulation and Notch/β-catenin/NF-κB signalling pathways.

Original languageEnglish
Pages (from-to)365-379
Number of pages15
JournalCancer Biomarkers
Volume30
Issue number4
DOIs
StatePublished - 1 Jan 2021
Externally publishedYes

Keywords

  • breast cancer
  • cell migration
  • LINC00052
  • transcriptome analysis

ASJC Scopus subject areas

  • Genetics
  • Oncology
  • Cancer Research

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