Abstract
The 130 kDa polypeptide appears to be the most susceptible of the polypeptides constituting the Bacillus thuringiensis ssp. israelensis δ-endotoxin to the proteases associated with sporulation. Stabilization of this polypeptide in the recently described K-S sporulation medium has been achieved through limiting its cleavage by removing contaminating proteases from the culture by washing. In this way, the complete δ-endotoxin profile is retained for studies on all of the constituent polypeptide fractions.
| Original language | English |
|---|---|
| Pages (from-to) | 377-381 |
| Number of pages | 5 |
| Journal | International Journal of Tropical Insect Science |
| Volume | 14 |
| Issue number | 3 |
| DOIs | |
| State | Published - 1993 |
Keywords
- Bacillus thuringiensis israelensis
- 130kDa polypeptide
- disappearance and stabilization
- K-S medium
- Bacillus ssp. thuringiensis israelensis
- polypeptide deolypeptide de poids moléculaire 130 kDa
- disparition et stabilisation
- milieu K-S