Interaction of a novel fluorescent analog of interferon-γ with transformed cells

Ayala Falach; Ilana Nathan; Stavanit Baram; Nurith Porat; Alexander Dvilansky; Abraham H. Parola

doi:10.1021/bc970063f

Interaction of a novel fluorescent analog of interferon-γ with transformed cells

Ayala Falach, Ilana Nathan, Stavanit Baram, Nurith Porat, Alexander Dvilansky, Abraham H. Parola

Research output: Contribution to journal › Article › peer-review

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Abstract

A fluorescent analog of human recombinant interferon-γ (IFN-γ) was prepared for the first time. The recovered pyrene-labeled IFN-γ (py-IFN-γ), with an estimated seven pyrene molecules per IFN-γ, retained over half of its original biological activity. Binding of py-IFN-γ to human amnion WISH cells showed appreciable enhancement in fluorescence polarization from 0.055 to 0.215 and in fluorescence lifetime from 56 to 80 ns. The ratio of the vibronic peaks did not change, indicating that the pyrene molecules remained in water environment even after binding. Py-IFN-γ provides a novel tool for unraveling the mechanism of the initial interaction between this antiproliferative lymphokine and its target, cancer cell membrane receptors. Its fluorescence could provide the means to follow receptor recycling when it occurs.

Original language	English
Pages (from-to)	459-465
Number of pages	7
Journal	Bioconjugate Chemistry
Volume	8
Issue number	4
DOIs	https://doi.org/10.1021/bc970063f
State	Published - 1 Jan 1997

ASJC Scopus subject areas

Biotechnology
Bioengineering
Biomedical Engineering
Pharmacology
Pharmaceutical Science
Organic Chemistry

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abstract = "A fluorescent analog of human recombinant interferon-γ (IFN-γ) was prepared for the first time. The recovered pyrene-labeled IFN-γ (py-IFN-γ), with an estimated seven pyrene molecules per IFN-γ, retained over half of its original biological activity. Binding of py-IFN-γ to human amnion WISH cells showed appreciable enhancement in fluorescence polarization from 0.055 to 0.215 and in fluorescence lifetime from 56 to 80 ns. The ratio of the vibronic peaks did not change, indicating that the pyrene molecules remained in water environment even after binding. Py-IFN-γ provides a novel tool for unraveling the mechanism of the initial interaction between this antiproliferative lymphokine and its target, cancer cell membrane receptors. Its fluorescence could provide the means to follow receptor recycling when it occurs.",

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T1 - Interaction of a novel fluorescent analog of interferon-γ with transformed cells

AU - Falach, Ayala

AU - Nathan, Ilana

AU - Baram, Stavanit

AU - Porat, Nurith

AU - Dvilansky, Alexander

AU - Parola, Abraham H.

PY - 1997/1/1

Y1 - 1997/1/1

N2 - A fluorescent analog of human recombinant interferon-γ (IFN-γ) was prepared for the first time. The recovered pyrene-labeled IFN-γ (py-IFN-γ), with an estimated seven pyrene molecules per IFN-γ, retained over half of its original biological activity. Binding of py-IFN-γ to human amnion WISH cells showed appreciable enhancement in fluorescence polarization from 0.055 to 0.215 and in fluorescence lifetime from 56 to 80 ns. The ratio of the vibronic peaks did not change, indicating that the pyrene molecules remained in water environment even after binding. Py-IFN-γ provides a novel tool for unraveling the mechanism of the initial interaction between this antiproliferative lymphokine and its target, cancer cell membrane receptors. Its fluorescence could provide the means to follow receptor recycling when it occurs.

AB - A fluorescent analog of human recombinant interferon-γ (IFN-γ) was prepared for the first time. The recovered pyrene-labeled IFN-γ (py-IFN-γ), with an estimated seven pyrene molecules per IFN-γ, retained over half of its original biological activity. Binding of py-IFN-γ to human amnion WISH cells showed appreciable enhancement in fluorescence polarization from 0.055 to 0.215 and in fluorescence lifetime from 56 to 80 ns. The ratio of the vibronic peaks did not change, indicating that the pyrene molecules remained in water environment even after binding. Py-IFN-γ provides a novel tool for unraveling the mechanism of the initial interaction between this antiproliferative lymphokine and its target, cancer cell membrane receptors. Its fluorescence could provide the means to follow receptor recycling when it occurs.

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Interaction of a novel fluorescent analog of interferon-γ with transformed cells

Abstract

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