Interactions between nitrate and ammonium during uptake by carob seedlings and the effect of the form of earlier nitrogen nutrition

Cristina Negev, S. Herman Lips, Maria Amélia Negev

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    14 Scopus citations

    Abstract

    Seedlings of carob (Ceratonia siliqua L. cv. Mulata) were used in two sets of experiments in order to evaluate; (1) the reciprocal effects of each nitrogen form on net uptake of nitrate and ammonium, and (2) the effect of earlier nitrogen nutrition on ammonium versus nitrate uptake. In the former group of experiments we studied the kinetics of nitrate and ammonium uptake as well as the interference of each of the two forms with net uptake of ammonium and nitrate by both nitrogen depleted and nitrogen fed carob seedlings. On the whole, nitrogen depletion led to increase in both affinity and Vmax of the system for both forms of nitrogen, at the same time as the effects of nitrate on uptake of ammonium and vice versa were concentration dependent. In the second group of experiments the effects of earlier nitrogen nutrition on nitrate and ammonium uptake were characterized, and in this case we observed that: (a) if only one form of N was supplied, ammonium was taken up in greater amounts than nitrate; (b) the presence of ammonium enhanced nitrate uptake; (c) ammonium uptake was inhibited by nitrate; (d) there was a significant effect of the earlier nitrogen nutrition on the response of the plants to a different nitrogen source. The latter was evident mainly as regards ammonium uptake by plants grown in ammonium nitrate. The interactions between nitrate and ammonium uptake systems are discussed on the basis of the adaptation to the nitrogen source during early growth.

    Original languageEnglish
    Pages (from-to)544-551
    Number of pages8
    JournalPhysiologia Plantarum
    Volume89
    Issue number3
    DOIs
    StatePublished - 1 Jan 1993

    Keywords

    • Ammonium
    • Ceratonia siliqua
    • carob
    • kinetics
    • nitrate
    • uptake systems

    ASJC Scopus subject areas

    • Physiology
    • Genetics
    • Plant Science
    • Cell Biology

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