Interfacial pH during mussel adhesive plaque formation

Nadine R.Martinez Rodriguez, Saurabh Das, Yair Kaufman, Jacob N. Israelachvili, J. Herbert Waite

Research output: Contribution to journalArticlepeer-review

82 Scopus citations

Abstract

Mussel (Mytilus californianus) adhesion to marine surfaces involves an intricate and adaptive synergy of molecules and spatio-temporal processes. Although the molecules, such as mussel foot proteins (mfps), are well characterized, deposition details remain vague and speculative. Developing methods for the precise surveillance of conditions that apply during mfp deposition would aid both in understanding mussel adhesion and translating this adhesion into useful technologies. To probe the interfacial pH at which mussels buffer the local environment during mfp deposition, a lipid bilayer with tethered pH-sensitive fluorochromes was assembled on mica. The interfacial pH during foot contact with modified mica ranged from 2.2 to 3.3, which is well below the seawater pH of ~ 8. The acidic pH serves multiple functions: it limits mfp-Dopa oxidation, thereby enabling the catecholic functionalities to adsorb to surface oxides by H-bonding and metal ion coordination, and provides a solubility switch for mfps, most of which aggregate at pH ≥ 7–8.

Original languageEnglish
Pages (from-to)221-227
Number of pages7
JournalBiofouling
Volume31
Issue number2
DOIs
StatePublished - 1 Jan 2015
Externally publishedYes

Keywords

  • Dopa
  • Mussel interfacial pH
  • Oregon Green® 488 DHPE
  • PH sensitive surface

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