Interleukin-1 receptor antagonist is produced by Sertoli cells in vitro

D. Zeyse, E. Lunenfeld, M. Beck, I. Prinsloo, M. Huleihel

Research output: Contribution to journalArticlepeer-review

41 Scopus citations


The interleukin-1 (IL-1) system has been suggested to be involved in the cell-to-cell cross-talk within the testis. To identify a testicular cell source of IL-1 receptor antagonist (IL-1ra), mouse Sertoli cells were isolated, purified, cultured, and examined for IL-1ra. Our investigation revealed that Sertoli cells produce large amounts of immunoreactive IL-1ra under basal culture conditions, as examined by enzyme-linked immunosorbent assays. Its expression can be induced, showing maximum concentrations after 8 h of stimulation. Lipopolysaccharide, as well as IL-1α and -β, were found to stimulate IL-1ra production in Sertoli cells. FSH is capable to induce IL-1ra production in Sertoli cells in a dose-dependent manner. Immunocytochemical staining confirmed the presence of IL-1ra in the cytoplasma of Sertoli cells. The presence of IL-1ra messenger RNA was demonstrated by RT-PCR analysis. Our results may help to better evaluate the IL-1 activity in the testis and may indicate the involvement of IL-1ra in the autocrine and paracrine regulation of testicular cell function.

Original languageEnglish
Pages (from-to)1521-1527
Number of pages7
Issue number4
StatePublished - 1 Jan 2000

ASJC Scopus subject areas

  • Endocrinology


Dive into the research topics of 'Interleukin-1 receptor antagonist is produced by Sertoli cells in vitro'. Together they form a unique fingerprint.

Cite this