Interleukin-2 production by cultured human granulosa cells

Raoul Orvieto, Andrea R. Genazzani, Felice Petraglia, Ariela Schwartz, Michal Shelef, Zion Ben-Rafael

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


Objective - To determine whether cultured human granulosa cells (GC) produce Interleukin (IL)-2 and whether this GC IL-2 production may be regulated by human chorionic gonadotropin (hCG). Materials and Methods - Human GC derived from preovulatory follicles during in vitro fertilization (IVF) cycles were cultured in the absence or presence of hCG. Interleukin-2 was measured in tissue-culture medium by an enzyme-linked immunosorbent assay (ELISA) system. Results - Cultured GCs were demonstrated to produce IL-2 in vitro. Moreover, a positive, but not statistically significant, dose-response effect was demonstrated between culture supernatant IL-2 levels and hCG concentration. Conclusions - Our data indicate that human GC produce IL-2 and that this production might be regulated by hCG. These results lend credence to the theory that IL-2 might be involved in the events leading to ovarian hyperstimulation syndrome (OHSS).

Original languageEnglish
Pages (from-to)297-300
Number of pages4
JournalInternational Journal of Fertility and Women's Medicine
Issue number5
StatePublished - 1 Dec 1997
Externally publishedYes


  • Granulosa cells
  • IL-2
  • Ovarian hyperstimulation syndrome

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology


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