Interleukin 8 in middle ear fluid during acute otitis media: Correlation with aetiology and bacterial eradication

Eugene Leibovitz, Ron Dagan, Joseph H. Laver, Lolita Piglansky, Simon Raiz, Miguel R. Abboud, Dan M. Fliss, Alberto Leiberman, Asher Barzilai

Research output: Contribution to journalArticlepeer-review

26 Scopus citations

Abstract

Objectives - To study the concentration of interleukin 8 (IL-8) in the middle ear fluid of children with acute otitis media and the association between IL-8 concentrations, aetiology of acute otitis media, and bacteriological sterilisation. Study design - Middle ear fluid was obtained by tympanocentesis at enrolment (day 1) and on day 4-5 in 81 children aged 3- 36 months with acute otitis media who received antibiotic treatment. IL-8 concentrations were measured by enzyme linked immunosorbent assay. Results - 101 samples were obtained on day 1 and 47 samples on day 4-5. 94 pathogens were isolated in 79 of 101 samples obtained on day 1: 56 Haemophilus influenzae, 35 Streptococcus pneumoniae, 2 Moraxella catarrhalis, and 1 Streptococcus pyogenes. Among 40 paired, initially culture positive samples, sterilisation was achieved on day 4-5 in 22 but not in 18 (13 H influenzae, 2 S pneumoniae, and 3 H influenzae and S pneumoniae concomitantly). IL-8 was detected in 96 of 101 and 46 of 47 samples obtained on days 1 and 4-5, respectively. Mean (SD) IL-8 concentration on day 1 was significantly higher in culture positive than in negative samples (12 636 (23 317) v 5920 (7080) pg/ml). In paired samples, IL-8 concentration fell in 12 of 22 ears in which sterilisation was achieved and in 9 of 21 ears with persistent or new infection. Mean (SD) IL-8 concentrations on day 4-5 were significantly higher in culture positive than in negative samples (15 420 (15 418) v 6695 (5092) pg/ml). Conclusions - Higher IL-8 concentrations are found in culture positive middle ear fluid in acute otitis media. Bacterial eradication is associated with a fall in these concentrations.

Original languageEnglish
Pages (from-to)165-168
Number of pages4
JournalArchives of Disease in Childhood
Volume82
Issue number2
DOIs
StatePublished - 30 May 2000
Externally publishedYes

Keywords

  • Haemophilus influenzae
  • Interleukin 8
  • Otitis media
  • Streptococcus pneumoniae

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

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