Intracellular persistence of chlamydial major outer-membrane protein, lipopolysaccharide and ribosomal RNA after non-productive infection of human monocytes with Chlamydia trachomatis serovar K

E. Schmitz, E. Nettelnbreker, H. Zeidler, M. Hammer, E. Manor, J. Wollenhaupt

Research output: Contribution to journalArticlepeer-review

36 Scopus citations

Abstract

The replication of Chlamydia trachomatis serovar K was studied in human peripheral blood monocytes (PBMo). The intracellular fate of the bacteria was examined by determining the presence of chlamydial major outer-membrane protein (MOMP), lipopolysaccharide (LPS) and ribosomal RNA (rRNA). In-vitro infection of PBMo with C. trachomatis serovar K was not productive. However, chlamydial MOMP antigen, demonstrated by immunofluorescence, was present in PBMo for up to 14 days. Infected monocytes also contained chlamydial rRNA, measured by in-vitro hybridisation, and LPS, measured by enzyme immunoassay, for up to 14 days. These data are compatible with the hypothesis that the infection of PBMo with C. trachomatis may play a role in the systemic distribution of chlamydial antigens, leading to systemic infections of urogenital chlamydial infection.

Original languageEnglish
Pages (from-to)278-285
Number of pages8
JournalJournal of Medical Microbiology
Volume38
Issue number4
DOIs
StatePublished - 1 Jan 1993
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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