TY - JOUR
T1 - Intracellular persistence of chlamydial major outer-membrane protein, lipopolysaccharide and ribosomal RNA after non-productive infection of human monocytes with Chlamydia trachomatis serovar K
AU - Schmitz, E.
AU - Nettelnbreker, E.
AU - Zeidler, H.
AU - Hammer, M.
AU - Manor, E.
AU - Wollenhaupt, J.
PY - 1993/1/1
Y1 - 1993/1/1
N2 - The replication of Chlamydia trachomatis serovar K was studied in human peripheral blood monocytes (PBMo). The intracellular fate of the bacteria was examined by determining the presence of chlamydial major outer-membrane protein (MOMP), lipopolysaccharide (LPS) and ribosomal RNA (rRNA). In-vitro infection of PBMo with C. trachomatis serovar K was not productive. However, chlamydial MOMP antigen, demonstrated by immunofluorescence, was present in PBMo for up to 14 days. Infected monocytes also contained chlamydial rRNA, measured by in-vitro hybridisation, and LPS, measured by enzyme immunoassay, for up to 14 days. These data are compatible with the hypothesis that the infection of PBMo with C. trachomatis may play a role in the systemic distribution of chlamydial antigens, leading to systemic infections of urogenital chlamydial infection.
AB - The replication of Chlamydia trachomatis serovar K was studied in human peripheral blood monocytes (PBMo). The intracellular fate of the bacteria was examined by determining the presence of chlamydial major outer-membrane protein (MOMP), lipopolysaccharide (LPS) and ribosomal RNA (rRNA). In-vitro infection of PBMo with C. trachomatis serovar K was not productive. However, chlamydial MOMP antigen, demonstrated by immunofluorescence, was present in PBMo for up to 14 days. Infected monocytes also contained chlamydial rRNA, measured by in-vitro hybridisation, and LPS, measured by enzyme immunoassay, for up to 14 days. These data are compatible with the hypothesis that the infection of PBMo with C. trachomatis may play a role in the systemic distribution of chlamydial antigens, leading to systemic infections of urogenital chlamydial infection.
UR - http://www.scopus.com/inward/record.url?scp=0027461595&partnerID=8YFLogxK
U2 - 10.1099/00222615-38-4-278
DO - 10.1099/00222615-38-4-278
M3 - Article
AN - SCOPUS:0027461595
SN - 0022-2615
VL - 38
SP - 278
EP - 285
JO - Journal of Medical Microbiology
JF - Journal of Medical Microbiology
IS - 4
ER -