Intracellular stores maintain stable cytosolic Ca2+ gradients in epithelial cells by active Ca2+ redistribution

A. Braiman; Z. Priel

doi:10.1054/ceca.2001.0245

Intracellular stores maintain stable cytosolic Ca²⁺ gradients in epithelial cells by active Ca²⁺ redistribution

A. Braiman, Z. Priel

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

A stable localized region of high calcium concentration near the plasma membrane has been postulated to exist as an outcome of prolonged calcium influx and to play a crucial role in regulation of cellular life. However, the mechanism supporting this phenomenon is a perplexing problem. We show here that a sustained localized region of high cytosolic Ca²⁺ concentration is formed near the plasma membrane. Calcium influx, calcium uptake by intracellular stores and calcium release from the stores are essential for this phenomenon. Our results strongly suggest that the mechanism of formation of stable calcium gradient near the plasma membrane involves a process of active redistribution - uptake of entering calcium into intracellular stores and its release from the stores toward the plasma membrane.

Original language	English
Pages (from-to)	361-371
Number of pages	11
Journal	Cell Calcium
Volume	30
Issue number	6
DOIs	https://doi.org/10.1054/ceca.2001.0245
State	Published - 1 Jan 2001

ASJC Scopus subject areas

Physiology
Molecular Biology
Cell Biology

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10.1054/ceca.2001.0245

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title = "Intracellular stores maintain stable cytosolic Ca2+ gradients in epithelial cells by active Ca2+ redistribution",

abstract = "A stable localized region of high calcium concentration near the plasma membrane has been postulated to exist as an outcome of prolonged calcium influx and to play a crucial role in regulation of cellular life. However, the mechanism supporting this phenomenon is a perplexing problem. We show here that a sustained localized region of high cytosolic Ca2+ concentration is formed near the plasma membrane. Calcium influx, calcium uptake by intracellular stores and calcium release from the stores are essential for this phenomenon. Our results strongly suggest that the mechanism of formation of stable calcium gradient near the plasma membrane involves a process of active redistribution - uptake of entering calcium into intracellular stores and its release from the stores toward the plasma membrane.",

author = "A. Braiman and Z. Priel",

note = "Funding Information: We thank Dr M. Hershfinkel for technical assistance, and Dr S. D. Silberberg and Dr A. Korngreen for their valuable comments. A. B. greatefully acknowledges the fellowship support of the Kreitman Foundation. This work was partially supported by a grant from the Israeli Science Foundation.",

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N1 - Funding Information: We thank Dr M. Hershfinkel for technical assistance, and Dr S. D. Silberberg and Dr A. Korngreen for their valuable comments. A. B. greatefully acknowledges the fellowship support of the Kreitman Foundation. This work was partially supported by a grant from the Israeli Science Foundation.

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N2 - A stable localized region of high calcium concentration near the plasma membrane has been postulated to exist as an outcome of prolonged calcium influx and to play a crucial role in regulation of cellular life. However, the mechanism supporting this phenomenon is a perplexing problem. We show here that a sustained localized region of high cytosolic Ca2+ concentration is formed near the plasma membrane. Calcium influx, calcium uptake by intracellular stores and calcium release from the stores are essential for this phenomenon. Our results strongly suggest that the mechanism of formation of stable calcium gradient near the plasma membrane involves a process of active redistribution - uptake of entering calcium into intracellular stores and its release from the stores toward the plasma membrane.

AB - A stable localized region of high calcium concentration near the plasma membrane has been postulated to exist as an outcome of prolonged calcium influx and to play a crucial role in regulation of cellular life. However, the mechanism supporting this phenomenon is a perplexing problem. We show here that a sustained localized region of high cytosolic Ca2+ concentration is formed near the plasma membrane. Calcium influx, calcium uptake by intracellular stores and calcium release from the stores are essential for this phenomenon. Our results strongly suggest that the mechanism of formation of stable calcium gradient near the plasma membrane involves a process of active redistribution - uptake of entering calcium into intracellular stores and its release from the stores toward the plasma membrane.

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Intracellular stores maintain stable cytosolic Ca²⁺ gradients in epithelial cells by active Ca²⁺ redistribution

Abstract

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