Intracerebroventricular antisense to inositol monophosphatase-1 reduces enzyme activity but does not affect Li-sensitive behavior

Alon Shamir, Galit Shaltiel, Galila Agam

Research output: Contribution to journalArticlepeer-review

2 Scopus citations

Abstract

Inositol monophosphatase (IMPase) inhibition is a hypothesized mechanism of action of lithium (Li). To test this hypothesis, the authors used the approach of antisense administration. Three days of an intracerebroventricular (icv) administration of 5 μg/20 μl 3′-phosphorothioated IMPA-1 antisense oligonucleotide sequence resulted in 20% reduction of rat periventricular IMPase activity. Li potentiates pilocarpine-induced seizures, because inhibition of IMPase leads to reduction in brain inositol levels. However, antisense-induced reduction in IMPase activity was not followed by seizures induced by subconvulsive pilocarpine doses.

Original languageEnglish
Pages (from-to)103-106
Number of pages4
JournalProgress in Neuro-Psychopharmacology and Biological Psychiatry
Volume26
Issue number1
DOIs
StatePublished - 18 Jan 2002
Externally publishedYes

Keywords

  • Antisense
  • Inositol monophosphatase
  • Lithium
  • Pilocarpine seizures

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