TY - JOUR
T1 - Intramolecular dynamics and conformational transition in proteins studied by biophysical labelling methods. Common and specific features of proteins from thermophylic micro-organisms
AU - Likhtenshtein, Gertz I.
AU - Febbraio, Ferdinando
AU - Nucci, Roberto
N1 - Funding Information:
This project was supported by Scientific Agreement 1988/1999 between the National Research Council (Italy) and Ministry of Science and the Arts (Israel).
PY - 2000/1/1
Y1 - 2000/1/1
N2 - A general survey is carried out on the theoretical grounds for methods of spin, luminescence and Moessbauer labels, as well as their application in the study of protein intramolecular dynamics. When combined, these methods allow the protein dynamics to be investigated within a wide range of correlation times (τc = 102-10-10 s) and amplitudes. The purposeful application of the methods to various proteins at different temperatures (30-330 K), water content, substrate addition, etc., revealed a number of dynamical processes and conformational transitions in proteins. The experiments indicated correlations between the local segmental mobility of protein globules in a nanosecond temporal scale and biochemical reactions, such as long-distance electron transfer, hydrolysis and photoreactions. The biophysical labelling methods results were analyzed together with the data on dynamics obtained using complementary physico-chemical methods and theoretical calculations. Special emphasis is given to recent results on proteins from thermophylic micro-organisms. The mechanisms of protein intramolecular dynamics and their role in the stability and functions of proteins and enzymes are discussed.
AB - A general survey is carried out on the theoretical grounds for methods of spin, luminescence and Moessbauer labels, as well as their application in the study of protein intramolecular dynamics. When combined, these methods allow the protein dynamics to be investigated within a wide range of correlation times (τc = 102-10-10 s) and amplitudes. The purposeful application of the methods to various proteins at different temperatures (30-330 K), water content, substrate addition, etc., revealed a number of dynamical processes and conformational transitions in proteins. The experiments indicated correlations between the local segmental mobility of protein globules in a nanosecond temporal scale and biochemical reactions, such as long-distance electron transfer, hydrolysis and photoreactions. The biophysical labelling methods results were analyzed together with the data on dynamics obtained using complementary physico-chemical methods and theoretical calculations. Special emphasis is given to recent results on proteins from thermophylic micro-organisms. The mechanisms of protein intramolecular dynamics and their role in the stability and functions of proteins and enzymes are discussed.
UR - http://www.scopus.com/inward/record.url?scp=0034257946&partnerID=8YFLogxK
U2 - 10.1016/S1386-1425(00)00290-0
DO - 10.1016/S1386-1425(00)00290-0
M3 - Article
AN - SCOPUS:0034257946
SN - 1386-1425
VL - 56
SP - 2011
EP - 2031
JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
IS - 10
ER -