Intranasal administration of gold nanoparticles designed to target the central nervous system: Fabrication and comparison between nanospheres and nanoprisms

Eduardo Gallardo-Toledo, Andreas Tapia-Arellano, Freddy Celis, Tomer Sinai, Marcelo Campos, Marcelo J. Kogan, Amnon C. Sintov

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The presence of the blood–brain barrier (BBB) limit gold nanoparticles (GNP) accumulation in central nervous system (CNS) after intravenous (IV) administration. The intranasal (IN) route has been suggested as a good strategy for circumventing the BBB. In this report, we used gold nanoprisms (78 nm) and nanospheres (47 nm), of comparable surface areas (8000 vs 7235 nm2) functionalized with a polyethylene glycol (PEG) and D1 peptide (GNPr-D1 and GNS-D1, respectively) to evaluate their delivery to the CNS after IN administration. Cell viability assay showed that GNPr-D1 and GNS-D1 were not cytotoxic at concentrations ranged between 0.05 and 0.5 nM. IN administration of GNPr-D1 and GNS-D1 demonstrated a significant difference between the two types of GNP, in which the latter reached the CNS in higher levels. Pharmacokinetic study showed that the peak brain level of gold was 0.75 h after IN administration of GNS-D1. After IN and IV administrations of GNS-D1, gold concentrations found in brain were 55 times higher via the IN route compared to IV administration. Data revealed that the IN route is more effective for targeting gold to the brain than IV administration. Finally, no significant difference was observed between the IN and IV routes in the distribution of GNS-D1 in the various brain areas.

Original languageEnglish
Article number119957
JournalInternational Journal of Pharmaceutics
Volume590
DOIs
StatePublished - 30 Nov 2020

Keywords

  • Central nervous system
  • Functionalized nanoparticles
  • Gold nanoparticles
  • Intranasal administration
  • Nose-to-brain delivery

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