Intrinsic Fluorescence of Metabolite Amyloids Allows Label-Free Monitoring of Their Formation and Dynamics in Live Cells

Shira Shaham-Niv, Zohar A. Arnon, Dorin Sade, Alexandra Lichtenstein, Evgeny A. Shirshin, Sofiya Kolusheva, Ehud Gazit

Research output: Contribution to journalArticlepeer-review

37 Scopus citations

Abstract

The formation of apoptosis-inducing amyloidal structures by metabolites has significantly extended the “amyloid hypothesis” to include non-proteinaceous, single metabolite building blocks. However, detection of metabolite assemblies is restricted compared to their larger protein-based counterparts owing to the hindrance of external labelling and limited immunohistochemical detection tools. Herein, we present the detection of the formation, dynamics, and cellular distribution of metabolite amyloid-like structures and provide mechanistic insights into the generation of supramolecular chromophores. Moreover, the intrinsic fluorescence properties allow the detection of metabolite assemblies in living cells without the use of external dyes. Altogether, this intrinsic fluorescence of metabolite assemblies further verifies their amyloidal nature, while providing an important tool for further investigation of their pathological role in inborn error of metabolism disorders.

Original languageEnglish
Pages (from-to)12444-12447
Number of pages4
JournalAngewandte Chemie - International Edition
Volume57
Issue number38
DOIs
StatePublished - 17 Sep 2018

Keywords

  • fluorescence
  • inborn errors of metabolism
  • live cell imaging
  • metabolites
  • self-assembly

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