Primenenie zondov, ispuskaiushchikh annigiliatsionnuiu zamedlennuiu fluorestsentsiiu, dlia issledovaniia model'nykh i biologicheskikh membran.

Translated title of the contribution: Investigation of model and biological membranes by probes emitting annihilated delayed fluorescence

V. M. Mekler, A. I. Kotel'nikov, G. I. Likhtenshteǐn

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Registration of the annihilated delayed fluorescence kinetics of anthracene, 1,2-benzanthracene, 1,2,5,6-dibenzanthracene, 7,12-dimethylbenzanthracene, pyrene, 1,2-benzpyrene, 3,4-benzpyrene, perylene, and other aromatic compounds under laser excitation permitted investigation of the quenching of excited triplet molecules by hydrophobic quenchers (ferrocene, nitroxide radicals). A long life-time of the triplet excited states (10(-4)-10(-5) s) makes it possible to investigate the probe-quencher interactions in liposome and natural membranes at the probe concentration of 10(-6)-10(-7) M and the ratio of quencher/lipid = = 10(-4). The method enables to investigate localization of the enzyme active centre (hemes, non-heme iron, flavine) and lipid matrix organization in the lipid-protein complexes.

Translated title of the contributionInvestigation of model and biological membranes by probes emitting annihilated delayed fluorescence
Original languageRussian
Pages (from-to)503-504
Number of pages2
JournalBiofizika
Volume28
Issue number3
StatePublished - 1 May 1983

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