Abstract
Registration of the annihilated delayed fluorescence kinetics of anthracene, 1,2-benzanthracene, 1,2,5,6-dibenzanthracene, 7,12-dimethylbenzanthracene, pyrene, 1,2-benzpyrene, 3,4-benzpyrene, perylene, and other aromatic compounds under laser excitation permitted investigation of the quenching of excited triplet molecules by hydrophobic quenchers (ferrocene, nitroxide radicals). A long life-time of the triplet excited states (10(-4)-10(-5) s) makes it possible to investigate the probe-quencher interactions in liposome and natural membranes at the probe concentration of 10(-6)-10(-7) M and the ratio of quencher/lipid = = 10(-4). The method enables to investigate localization of the enzyme active centre (hemes, non-heme iron, flavine) and lipid matrix organization in the lipid-protein complexes.
Translated title of the contribution | Investigation of model and biological membranes by probes emitting annihilated delayed fluorescence |
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Original language | Russian |
Pages (from-to) | 503-504 |
Number of pages | 2 |
Journal | Biofizika |
Volume | 28 |
Issue number | 3 |
State | Published - 1 May 1983 |