Investigations of antimicrobial peptides in planar film systems

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56 Scopus citations


Planar systems - monolayers and films - constitute a useful platform for studying membrane-active peptides. Here, we summarize varied approaches for studying peptide organization and peptide-lipid interactions at the air/water interface, and focus on three representative antimicrobial membrane-associated peptides-alamethicin, gramicidin, and valinomycin. Experimental data, specifically surface pressure/area isotherms and Brewster angle microscopy images, provided information on peptide association and the effects of the lipid monolayers on peptide surface organization. In general, film analysis emphasized the effects of lipid layers in promoting peptide association and aggregation at the air/water interface. Importantly, the data demonstrated that in many cases peptide domains are phase-separated within the phospholipid monolayers, suggesting that this behavior contributes to the biological actions of membrane-active antimicrobial peptides.

Original languageEnglish
Pages (from-to)1393-1407
Number of pages15
JournalBiochimica et Biophysica Acta - Biomembranes
Issue number9
StatePublished - 1 Sep 2006


  • Brewster angle microscopy
  • Langmuir monolayer
  • Peptide film
  • Peptide-membrane interaction

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology


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