Involvement of nitric oxide in the promotion of cell survival by ceramide 1-phosphate

Patricia Gangoiti; Maria H. Granado; Lide Arana; Alberto Ouro; Antonio Gómez-Muñoz

doi:10.1016/j.febslet.2008.05.027

Involvement of nitric oxide in the promotion of cell survival by ceramide 1-phosphate

Patricia Gangoiti, Maria H. Granado, Lide Arana, Alberto Ouro, Antonio Gómez-Muñoz

Research output: Contribution to journal › Article › peer-review

37 Scopus citations

Abstract

Macrophages play vital roles in inflammatory responses, and their number at sites of inflammation is strictly regulated by cell death and division. Here, we demonstrate that production of nitric oxide (NO) is a major mechanism whereby ceramide-1-phosphate (C1P) blocks apoptosis in macrophages. However, NO failed to stimulate macrophage proliferation. The prosurvival effect of C1P was blocked by inhibitors of inducible NO synthase. The antiapoptotic effect of C1P was also blocked by phosphatidylinositol 3-kinase or nuclear factor-kappa B inhibitors. Moreover, NO reversed the inhibitory effect of C1P on acid sphingomyelinase, but the prosurvival effect of C1P was independent of this action.

Original language	English
Pages (from-to)	2263-2269
Number of pages	7
Journal	FEBS Letters
Volume	582
Issue number	15
DOIs	https://doi.org/10.1016/j.febslet.2008.05.027
State	Published - 25 Jun 2008
Externally published	Yes

Keywords

Apoptosis
Ceramide 1-phosphate
NF-κB
Nitric oxide
PI3-kinase
Sphingolipids

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/j.febslet.2008.05.027

Cite this

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title = "Involvement of nitric oxide in the promotion of cell survival by ceramide 1-phosphate",

abstract = "Macrophages play vital roles in inflammatory responses, and their number at sites of inflammation is strictly regulated by cell death and division. Here, we demonstrate that production of nitric oxide (NO) is a major mechanism whereby ceramide-1-phosphate (C1P) blocks apoptosis in macrophages. However, NO failed to stimulate macrophage proliferation. The prosurvival effect of C1P was blocked by inhibitors of inducible NO synthase. The antiapoptotic effect of C1P was also blocked by phosphatidylinositol 3-kinase or nuclear factor-kappa B inhibitors. Moreover, NO reversed the inhibitory effect of C1P on acid sphingomyelinase, but the prosurvival effect of C1P was independent of this action.",

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AU - Gangoiti, Patricia

AU - Granado, Maria H.

AU - Arana, Lide

AU - Ouro, Alberto

AU - Gómez-Muñoz, Antonio

PY - 2008/6/25

Y1 - 2008/6/25

N2 - Macrophages play vital roles in inflammatory responses, and their number at sites of inflammation is strictly regulated by cell death and division. Here, we demonstrate that production of nitric oxide (NO) is a major mechanism whereby ceramide-1-phosphate (C1P) blocks apoptosis in macrophages. However, NO failed to stimulate macrophage proliferation. The prosurvival effect of C1P was blocked by inhibitors of inducible NO synthase. The antiapoptotic effect of C1P was also blocked by phosphatidylinositol 3-kinase or nuclear factor-kappa B inhibitors. Moreover, NO reversed the inhibitory effect of C1P on acid sphingomyelinase, but the prosurvival effect of C1P was independent of this action.

AB - Macrophages play vital roles in inflammatory responses, and their number at sites of inflammation is strictly regulated by cell death and division. Here, we demonstrate that production of nitric oxide (NO) is a major mechanism whereby ceramide-1-phosphate (C1P) blocks apoptosis in macrophages. However, NO failed to stimulate macrophage proliferation. The prosurvival effect of C1P was blocked by inhibitors of inducible NO synthase. The antiapoptotic effect of C1P was also blocked by phosphatidylinositol 3-kinase or nuclear factor-kappa B inhibitors. Moreover, NO reversed the inhibitory effect of C1P on acid sphingomyelinase, but the prosurvival effect of C1P was independent of this action.

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KW - Ceramide 1-phosphate

KW - NF-κB

KW - Nitric oxide

KW - PI3-kinase

KW - Sphingolipids

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Involvement of nitric oxide in the promotion of cell survival by ceramide 1-phosphate

Abstract

Keywords

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