CONFERENCE PROCEEDINGS, NON PEER REVIEWED Swollenin, a protein first characterized in the saprophytic fungus Trichoderma reesei (Saloheimo et al., 2000), contains an N-terminal Carbohydrate Binding Module Family 1 domain (CBD) and a C-terminal expansin-like domain. This protein was identified by LC/MS among many other cellulolytic proteins secreted in the co-culture hydroponics medium of cucumber seedlings and T. asperellum, a well known biocontrol agent and inducer of plant defense responses. Trichoderma transformants overexpressing this protein showed a remarkably increased ability to colonize cucumber roots. Root colonization rates were reduced in transformants silenced in swollenin gene expression. These same transformants were capable to overgrow Rhizoctonia solani but not Pythium aphanidermatum in plate confrontations assays. Real time PCR analysis could show that the swollenin gene is up-regulated during mycoparasitic interaction with P. aphanidermatum but not with R. solani.
|State||Published - 2009|