Involvement of two-pore channels in hydrogen peroxide-induced increase in the level of calcium ions in the cytoplasm of human umbilical vein endothelial cells

P. V. Avdonin; A. D. Nadeev; E. B. Tsitrin; A. A. Tsitrina; P. P. Avdonin; G. Yu Mironova; I. L. Zharkikh; N. V. Goncharov

doi:10.1134/S1607672917030152

Involvement of two-pore channels in hydrogen peroxide-induced increase in the level of calcium ions in the cytoplasm of human umbilical vein endothelial cells

P. V. Avdonin, A. D. Nadeev, E. B. Tsitrin, A. A. Tsitrina, P. P. Avdonin, G. Yu Mironova, I. L. Zharkikh, N. V. Goncharov

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Hydrogen peroxide at concentrations below cytotoxic ones causes an increase in the cytoplasmic calcium concentration in human umbilical vein endothelial cells as a result of calcium release from intracellular stores. Two-pore calcium channel blocker trans-NED19 partially suppresses the increase in the level of calcium ions in the cells in response to the addition of hydrogen peroxide. The staining of endothelial cells with the fluorescent stereoisomer cis-NED19 and LysoTracker confirmed the localization of two-pore calcium channels in lysosomes and endolysosomal vesicles.

Original language	English
Pages (from-to)	209-212
Number of pages	4
Journal	Doklady Biochemistry and Biophysics
Volume	474
Issue number	1
DOIs	https://doi.org/10.1134/S1607672917030152
State	Published - 1 May 2017
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Biochemistry
General Chemistry

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Avdonin, P. V., Nadeev, A. D., Tsitrin, E. B., Tsitrina, A. A., Avdonin, P. P., Mironova, G. Y., Zharkikh, I. L., & Goncharov, N. V. (2017). Involvement of two-pore channels in hydrogen peroxide-induced increase in the level of calcium ions in the cytoplasm of human umbilical vein endothelial cells. Doklady Biochemistry and Biophysics, 474(1), 209-212. https://doi.org/10.1134/S1607672917030152

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title = "Involvement of two-pore channels in hydrogen peroxide-induced increase in the level of calcium ions in the cytoplasm of human umbilical vein endothelial cells",

abstract = "Hydrogen peroxide at concentrations below cytotoxic ones causes an increase in the cytoplasmic calcium concentration in human umbilical vein endothelial cells as a result of calcium release from intracellular stores. Two-pore calcium channel blocker trans-NED19 partially suppresses the increase in the level of calcium ions in the cells in response to the addition of hydrogen peroxide. The staining of endothelial cells with the fluorescent stereoisomer cis-NED19 and LysoTracker confirmed the localization of two-pore calcium channels in lysosomes and endolysosomal vesicles.",

author = "Avdonin, {P. V.} and Nadeev, {A. D.} and Tsitrin, {E. B.} and Tsitrina, {A. A.} and Avdonin, {P. P.} and Mironova, {G. Yu} and Zharkikh, {I. L.} and Goncharov, {N. V.}",

note = "Publisher Copyright: {\textcopyright} 2017, Pleiades Publishing, Ltd.",

year = "2017",

month = may,

day = "1",

doi = "10.1134/S1607672917030152",

language = "English",

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journal = "Doklady Biochemistry and Biophysics",

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Avdonin, PV, Nadeev, AD, Tsitrin, EB, Tsitrina, AA, Avdonin, PP, Mironova, GY, Zharkikh, IL & Goncharov, NV 2017, 'Involvement of two-pore channels in hydrogen peroxide-induced increase in the level of calcium ions in the cytoplasm of human umbilical vein endothelial cells', Doklady Biochemistry and Biophysics, vol. 474, no. 1, pp. 209-212. https://doi.org/10.1134/S1607672917030152

TY - JOUR

T1 - Involvement of two-pore channels in hydrogen peroxide-induced increase in the level of calcium ions in the cytoplasm of human umbilical vein endothelial cells

AU - Avdonin, P. V.

AU - Nadeev, A. D.

AU - Tsitrin, E. B.

AU - Tsitrina, A. A.

AU - Avdonin, P. P.

AU - Mironova, G. Yu

AU - Zharkikh, I. L.

AU - Goncharov, N. V.

PY - 2017/5/1

Y1 - 2017/5/1

N2 - Hydrogen peroxide at concentrations below cytotoxic ones causes an increase in the cytoplasmic calcium concentration in human umbilical vein endothelial cells as a result of calcium release from intracellular stores. Two-pore calcium channel blocker trans-NED19 partially suppresses the increase in the level of calcium ions in the cells in response to the addition of hydrogen peroxide. The staining of endothelial cells with the fluorescent stereoisomer cis-NED19 and LysoTracker confirmed the localization of two-pore calcium channels in lysosomes and endolysosomal vesicles.

AB - Hydrogen peroxide at concentrations below cytotoxic ones causes an increase in the cytoplasmic calcium concentration in human umbilical vein endothelial cells as a result of calcium release from intracellular stores. Two-pore calcium channel blocker trans-NED19 partially suppresses the increase in the level of calcium ions in the cells in response to the addition of hydrogen peroxide. The staining of endothelial cells with the fluorescent stereoisomer cis-NED19 and LysoTracker confirmed the localization of two-pore calcium channels in lysosomes and endolysosomal vesicles.

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AN - SCOPUS:85025101688

SN - 1607-6729

VL - 474

SP - 209

EP - 212

JO - Doklady Biochemistry and Biophysics

JF - Doklady Biochemistry and Biophysics

IS - 1

ER -

Involvement of two-pore channels in hydrogen peroxide-induced increase in the level of calcium ions in the cytoplasm of human umbilical vein endothelial cells

Abstract

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