Irreversible adsorption of triple-helical soluble collagen monomers from solution to glass and other surfaces

G. Penners; Z. Priel; A. Silberberg

doi:10.1016/0021-9797(81)90202-2

Irreversible adsorption of triple-helical soluble collagen monomers from solution to glass and other surfaces

G. Penners, Z. Priel, A. Silberberg

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

The adsorption from various solutions of triple-helical soluble collagen monomers to solid surfaces was studied by labeling the collagen with ¹²⁵1. Adsorption to glass, siliconized glass, and Teflon, from aqueous solutions at various pH and ionic strength, was determined at collagen concentrations from 2 to 25 μg/ml. Adsorption was shown to be irreversible and little dependent on pH and ionic strength but increasing enormously as the surface is made more hydrophobic. Surface denaturation of the collagen by heat results in a substantial loss of material. The kinetics of adsorption suggest that the adsorption process may be selective and that not all collagen molecules which reach the surface are immediately adsorbed. Checking these results with earlier measurements of adsorbed layer thickness, a model for collagen adsorption is proposed.

Original language	English
Pages (from-to)	437-444
Number of pages	8
Journal	Journal of Colloid and Interface Science
Volume	80
Issue number	2
DOIs	https://doi.org/10.1016/0021-9797(81)90202-2
State	Published - 1 Jan 1981

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Biomaterials
Surfaces, Coatings and Films
Colloid and Surface Chemistry

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10.1016/0021-9797(81)90202-2

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title = "Irreversible adsorption of triple-helical soluble collagen monomers from solution to glass and other surfaces",

abstract = "The adsorption from various solutions of triple-helical soluble collagen monomers to solid surfaces was studied by labeling the collagen with 1251. Adsorption to glass, siliconized glass, and Teflon, from aqueous solutions at various pH and ionic strength, was determined at collagen concentrations from 2 to 25 μg/ml. Adsorption was shown to be irreversible and little dependent on pH and ionic strength but increasing enormously as the surface is made more hydrophobic. Surface denaturation of the collagen by heat results in a substantial loss of material. The kinetics of adsorption suggest that the adsorption process may be selective and that not all collagen molecules which reach the surface are immediately adsorbed. Checking these results with earlier measurements of adsorbed layer thickness, a model for collagen adsorption is proposed.",

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AU - Penners, G.

AU - Priel, Z.

AU - Silberberg, A.

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Y1 - 1981/1/1

N2 - The adsorption from various solutions of triple-helical soluble collagen monomers to solid surfaces was studied by labeling the collagen with 1251. Adsorption to glass, siliconized glass, and Teflon, from aqueous solutions at various pH and ionic strength, was determined at collagen concentrations from 2 to 25 μg/ml. Adsorption was shown to be irreversible and little dependent on pH and ionic strength but increasing enormously as the surface is made more hydrophobic. Surface denaturation of the collagen by heat results in a substantial loss of material. The kinetics of adsorption suggest that the adsorption process may be selective and that not all collagen molecules which reach the surface are immediately adsorbed. Checking these results with earlier measurements of adsorbed layer thickness, a model for collagen adsorption is proposed.

AB - The adsorption from various solutions of triple-helical soluble collagen monomers to solid surfaces was studied by labeling the collagen with 1251. Adsorption to glass, siliconized glass, and Teflon, from aqueous solutions at various pH and ionic strength, was determined at collagen concentrations from 2 to 25 μg/ml. Adsorption was shown to be irreversible and little dependent on pH and ionic strength but increasing enormously as the surface is made more hydrophobic. Surface denaturation of the collagen by heat results in a substantial loss of material. The kinetics of adsorption suggest that the adsorption process may be selective and that not all collagen molecules which reach the surface are immediately adsorbed. Checking these results with earlier measurements of adsorbed layer thickness, a model for collagen adsorption is proposed.

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Irreversible adsorption of triple-helical soluble collagen monomers from solution to glass and other surfaces

Abstract

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