The adsorption from various solutions of triple-helical soluble collagen monomers to solid surfaces was studied by labeling the collagen with 1251. Adsorption to glass, siliconized glass, and Teflon, from aqueous solutions at various pH and ionic strength, was determined at collagen concentrations from 2 to 25 μg/ml. Adsorption was shown to be irreversible and little dependent on pH and ionic strength but increasing enormously as the surface is made more hydrophobic. Surface denaturation of the collagen by heat results in a substantial loss of material. The kinetics of adsorption suggest that the adsorption process may be selective and that not all collagen molecules which reach the surface are immediately adsorbed. Checking these results with earlier measurements of adsorbed layer thickness, a model for collagen adsorption is proposed.
ASJC Scopus subject areas
- Electronic, Optical and Magnetic Materials
- Surfaces, Coatings and Films
- Colloid and Surface Chemistry