Ischemic poison lysophosphatidylcholine modifies heart sodium channels gating inducing long-lasting bursts of openings

N. A. Burnashev; A. I. Undrovinas; I. A. Fleidervish; L. V. Rosenshtraukh

doi:10.1007/BF00373151

Ischemic poison lysophosphatidylcholine modifies heart sodium channels gating inducing long-lasting bursts of openings

N. A. Burnashev, A. I. Undrovinas, I. A. Fleidervish, L. V. Rosenshtraukh

Research output: Contribution to journal › Article › peer-review

45 Scopus citations

Abstract

The effects of lysophosphatidylcholine (LPC) on Na channels in inside-out patches of adult rat ventricular cells using the patch-clamp technique have been investigated. Application of LPC (9-25 μM) from the inner side of membrane for 4-15 min caused a reduction of averaged Na current (I_Na) peak and prolonged the time course of inactivation in the potential range of -50 to -10 mV. Analysis of single channel behaviour revealed that after 30-50 min of exposure, in addition to normally functioning Na channels with short openings, LPC induced longlasting bursts of Na channel openings (up to the 300 ms duration of the test pulses). This resulted in an appearance of noninactivated component of I_Na. The slope conductance of these modified channels remained the same as in control (11.3 pS - control; 11.6 pS - LPC-treated). The dwell time for modified channels increased significantly.

Original language	English
Pages (from-to)	124-126
Number of pages	3
Journal	Pflugers Archiv European Journal of Physiology
Volume	415
Issue number	1
DOIs	https://doi.org/10.1007/BF00373151
State	Published - 1 Jan 1989
Externally published	Yes

Keywords

Sodium channels
heart cells
lysophosphatidylcholine

ASJC Scopus subject areas

Physiology
Clinical Biochemistry
Physiology (medical)

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10.1007/BF00373151

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title = "Ischemic poison lysophosphatidylcholine modifies heart sodium channels gating inducing long-lasting bursts of openings",

abstract = "The effects of lysophosphatidylcholine (LPC) on Na channels in inside-out patches of adult rat ventricular cells using the patch-clamp technique have been investigated. Application of LPC (9-25 μM) from the inner side of membrane for 4-15 min caused a reduction of averaged Na current (INa) peak and prolonged the time course of inactivation in the potential range of -50 to -10 mV. Analysis of single channel behaviour revealed that after 30-50 min of exposure, in addition to normally functioning Na channels with short openings, LPC induced longlasting bursts of Na channel openings (up to the 300 ms duration of the test pulses). This resulted in an appearance of noninactivated component of INa. The slope conductance of these modified channels remained the same as in control (11.3 pS - control; 11.6 pS - LPC-treated). The dwell time for modified channels increased significantly.",

keywords = "Sodium channels, heart cells, lysophosphatidylcholine",

author = "Burnashev, {N. A.} and Undrovinas, {A. I.} and Fleidervish, {I. A.} and Rosenshtraukh, {L. V.}",

year = "1989",

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doi = "10.1007/BF00373151",

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T1 - Ischemic poison lysophosphatidylcholine modifies heart sodium channels gating inducing long-lasting bursts of openings

AU - Burnashev, N. A.

AU - Undrovinas, A. I.

AU - Fleidervish, I. A.

AU - Rosenshtraukh, L. V.

PY - 1989/1/1

Y1 - 1989/1/1

N2 - The effects of lysophosphatidylcholine (LPC) on Na channels in inside-out patches of adult rat ventricular cells using the patch-clamp technique have been investigated. Application of LPC (9-25 μM) from the inner side of membrane for 4-15 min caused a reduction of averaged Na current (INa) peak and prolonged the time course of inactivation in the potential range of -50 to -10 mV. Analysis of single channel behaviour revealed that after 30-50 min of exposure, in addition to normally functioning Na channels with short openings, LPC induced longlasting bursts of Na channel openings (up to the 300 ms duration of the test pulses). This resulted in an appearance of noninactivated component of INa. The slope conductance of these modified channels remained the same as in control (11.3 pS - control; 11.6 pS - LPC-treated). The dwell time for modified channels increased significantly.

AB - The effects of lysophosphatidylcholine (LPC) on Na channels in inside-out patches of adult rat ventricular cells using the patch-clamp technique have been investigated. Application of LPC (9-25 μM) from the inner side of membrane for 4-15 min caused a reduction of averaged Na current (INa) peak and prolonged the time course of inactivation in the potential range of -50 to -10 mV. Analysis of single channel behaviour revealed that after 30-50 min of exposure, in addition to normally functioning Na channels with short openings, LPC induced longlasting bursts of Na channel openings (up to the 300 ms duration of the test pulses). This resulted in an appearance of noninactivated component of INa. The slope conductance of these modified channels remained the same as in control (11.3 pS - control; 11.6 pS - LPC-treated). The dwell time for modified channels increased significantly.

KW - Sodium channels

KW - heart cells

KW - lysophosphatidylcholine

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ER -

Ischemic poison lysophosphatidylcholine modifies heart sodium channels gating inducing long-lasting bursts of openings

Abstract

Keywords

ASJC Scopus subject areas

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