Isolation and characterization of a functional promoter from Nitrosomonas europaea

A. Nejidat; O. Spector; A. Abeliovich

doi:10.1016/0378-1097(96)00011-0

Isolation and characterization of a functional promoter from Nitrosomonas europaea

A. Nejidat, O. Spector, A. Abeliovich

Research output: Contribution to journal › Article › peer-review

4 Scopus citations

Abstract

A functional promoter from the obligate autotrophic ammonia oxidizing bacterium Nitrosomonas europaea was identified by expression in Escherichia coli, using a promoterless reporter gene. Transcription initiation site of this promoter was determined by primer extension analysis. The sequences at - 35 and - 10 have low similarity to the - 10/- 35 consensus sequence of known prokaryotic promoters.

Original language	English
Pages (from-to)	9-12
Number of pages	4
Journal	FEMS Microbiology Letters
Volume	137
Issue number	1
DOIs	https://doi.org/10.1016/0378-1097(96)00011-0
State	Published - 15 Mar 1996

Keywords

Nitrifying bacteria
Nitrosomonas europaea
Promoter analysis
Transcription initiation

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

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10.1016/0378-1097(96)00011-0

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title = "Isolation and characterization of a functional promoter from Nitrosomonas europaea",

abstract = "A functional promoter from the obligate autotrophic ammonia oxidizing bacterium Nitrosomonas europaea was identified by expression in Escherichia coli, using a promoterless reporter gene. Transcription initiation site of this promoter was determined by primer extension analysis. The sequences at - 35 and - 10 have low similarity to the - 10/- 35 consensus sequence of known prokaryotic promoters.",

keywords = "Nitrifying bacteria, Nitrosomonas europaea, Promoter analysis, Transcription initiation",

author = "A. Nejidat and O. Spector and A. Abeliovich",

note = "Funding Information: We thank Prof. Y. Heimer and Mr. S. Barak for reading the manuscript.T his work was supportedb y the Rashi Foundation and an Eshcol grant from the Israeli Ministry of Science and Technology to A.N.",

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T1 - Isolation and characterization of a functional promoter from Nitrosomonas europaea

AU - Nejidat, A.

AU - Spector, O.

AU - Abeliovich, A.

N1 - Funding Information: We thank Prof. Y. Heimer and Mr. S. Barak for reading the manuscript.T his work was supportedb y the Rashi Foundation and an Eshcol grant from the Israeli Ministry of Science and Technology to A.N.

PY - 1996/3/15

Y1 - 1996/3/15

N2 - A functional promoter from the obligate autotrophic ammonia oxidizing bacterium Nitrosomonas europaea was identified by expression in Escherichia coli, using a promoterless reporter gene. Transcription initiation site of this promoter was determined by primer extension analysis. The sequences at - 35 and - 10 have low similarity to the - 10/- 35 consensus sequence of known prokaryotic promoters.

AB - A functional promoter from the obligate autotrophic ammonia oxidizing bacterium Nitrosomonas europaea was identified by expression in Escherichia coli, using a promoterless reporter gene. Transcription initiation site of this promoter was determined by primer extension analysis. The sequences at - 35 and - 10 have low similarity to the - 10/- 35 consensus sequence of known prokaryotic promoters.

KW - Nitrifying bacteria

KW - Nitrosomonas europaea

KW - Promoter analysis

KW - Transcription initiation

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U2 - 10.1016/0378-1097(96)00011-0

DO - 10.1016/0378-1097(96)00011-0

M3 - Article

AN - SCOPUS:0029878370

SN - 0378-1097

VL - 137

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EP - 12

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1

ER -

Isolation and characterization of a functional promoter from Nitrosomonas europaea

Abstract

Keywords

ASJC Scopus subject areas

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