Isolation and characterization of endophytic fungi having plant growth promotion traits that biosynthesizes bacosides and withanolides under in vitro conditions

Sumit K. Soni, Rakshapal Singh, Nem K. Ngpoore, Abhishek Niranjan, Purnima Singh, Aradhana Mishra, Sudeep Tiwari

Research output: Contribution to journalArticlepeer-review

15 Scopus citations

Abstract

Endophytes are regarded with immense potentials in terms of plant growth promoting (PGP) elicitors and mimicking secondary metabolites of medicinal importance. Here in the present study, we explored Bacopa monnieri plants to isolate, identify fungal endophytes with PGP elicitation potentials, and investigate secretion of secondary metabolites such as bacoside and withanolide content under in vitro conditions. Three fungal endophytes isolated (out of 40 saponin producing isolates) from leaves of B. monnieri were examined for in vitro biosynthesis of bacosides. On morphological, biochemical, and molecular identification (ITS gene sequencing), the isolated strains SUBL33, SUBL51, and SUBL206 were identified as Nigrospora oryzae (MH071153), Alternaria alternata (MH071155), and Aspergillus terreus (MH071154) respectively. Among these strains, SUBL33 produced highest quantity of Bacoside A3 (4093 μg mL−1), Jujubogenin isomer of Bacopasaponin C (65,339 μg mL−1), and Bacopasaponin C (1325 μg mL−1) while Bacopaside II (13,030 μg mL−1) was produced by SUBL51 maximally. Moreover, these aforementioned strains also produced detectable concentration of withanolides—Withaferrin A, Withanolide A (480 μg mL−1), and Withanolide B (1024 μg mL−1) respectively. However, Withanolide A was not detected in the secondary metabolites of strain SUBL51. To best of our knowledge, the present study is first reports of Nigrospora oryzae as an endophyte in B. monnieri with potentials of biosynthesis of economically important phytomolecules under in vitro conditions.

Original languageEnglish
Pages (from-to)1791-1805
Number of pages15
JournalBrazilian Journal of Microbiology
Volume52
Issue number4
DOIs
StatePublished - 1 Dec 2021

Keywords

  • Bacopa monnieri
  • Bacosides
  • Endophytes
  • In vitro biosynthesis
  • Medicinal plants
  • Saponin
  • Withanolides

ASJC Scopus subject areas

  • Microbiology

Fingerprint

Dive into the research topics of 'Isolation and characterization of endophytic fungi having plant growth promotion traits that biosynthesizes bacosides and withanolides under in vitro conditions'. Together they form a unique fingerprint.

Cite this