TY - JOUR
T1 - Isolation and characterization of Kingella negevensis sp. Nov., a novel kingella species detected in a healthy paediatric population
AU - El Houmami, Nawal
AU - Bakour, Sofiane
AU - Bzdrenga, Janek
AU - Rathored, Jaishiram
AU - Seligmann, Hervé
AU - Robert, Catherine
AU - Armstrong, Nicholas
AU - Schrenzel, Jacques
AU - Raoult, Didier
AU - Yagupsky, Pablo
AU - Fournier, Pierre Edouard
N1 - Funding Information:
This work was supported bya the Institut Hospitalo-Universitaire Méditerranée Infection foundation, and has been carried out thanks to the support of the A*MIDEX project (no. ANR-11-IDEX-0001-02) funded by the ‘Investissements d’Avenir’ French Government programme, managed by the French National Research Agency (ANR).
Publisher Copyright:
© 2017 IUMS.
PY - 2017/7/1
Y1 - 2017/7/1
N2 - We herein report the isolation and characterization of 21 Gram-stain-negative strains cultivated from the oropharynx of healthy children in Israel and Switzerland. Initially described as small colony variants of Kingella kingae, phenotypic analysis, biochemical analysis, phylogenetic analysis based on sequencing of the partial 16S rRNA gene and five housekeeping genes (abcZ, adk, G6PD, groEL and recA), and whole genome sequencing and comparison between members of the genera Kingella and Neisseria provided evidence for assigning them to the genus Kingella. Cellular fatty acids included important amounts of C12:0, C14:0, C16:0 and C16:1n7. Digital DNA–DNA hybridization between the isolates Sch538T and K. kingae ATCC 23330T revealed relatedness of 19.9 %. Comparative analysis of 16S rRNA gene sequences available in GenBank allowed matches to strains isolated in the USA, suggesting a wider geographical distribution. A novel species named Kingella negevensis sp. nov. is proposed, as most strains have been isolated in the Negev, a desert region of southern Israel. The type strain is Sch538T (=CCUG 69806T=CSUR P957).
AB - We herein report the isolation and characterization of 21 Gram-stain-negative strains cultivated from the oropharynx of healthy children in Israel and Switzerland. Initially described as small colony variants of Kingella kingae, phenotypic analysis, biochemical analysis, phylogenetic analysis based on sequencing of the partial 16S rRNA gene and five housekeeping genes (abcZ, adk, G6PD, groEL and recA), and whole genome sequencing and comparison between members of the genera Kingella and Neisseria provided evidence for assigning them to the genus Kingella. Cellular fatty acids included important amounts of C12:0, C14:0, C16:0 and C16:1n7. Digital DNA–DNA hybridization between the isolates Sch538T and K. kingae ATCC 23330T revealed relatedness of 19.9 %. Comparative analysis of 16S rRNA gene sequences available in GenBank allowed matches to strains isolated in the USA, suggesting a wider geographical distribution. A novel species named Kingella negevensis sp. nov. is proposed, as most strains have been isolated in the Negev, a desert region of southern Israel. The type strain is Sch538T (=CCUG 69806T=CSUR P957).
KW - Genomics
KW - Kingella kingae
KW - Kingella negevensis
KW - Neisseriaceae
KW - Paediatrics
UR - http://www.scopus.com/inward/record.url?scp=85021856842&partnerID=8YFLogxK
U2 - 10.1099/ijsem.0.001957
DO - 10.1099/ijsem.0.001957
M3 - Article
AN - SCOPUS:85021856842
VL - 67
SP - 2370
EP - 2376
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
SN - 1466-5026
IS - 7
ER -