Isolation and characterization of the plasma membrane by two-phase partitioning from the alkalophilic cyanobacterium Spirulina platensis

Chang Hong Xu, Ali Nejidat, Shimshon Belkin, Sammy Boussiba

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Partition in aqueous dextran-polyethylene glycol two-phase system was used to isolate the plasma membranes from the alkalophilic cyanobacterium Spirulina platensis. The upper phase contained a colorless membranes obtained in relatively short time, 3-4 h. This fraction had a different protein profile than that of the thylakoid fraction obtained in the lower phase. It did not contain cytochrome c-oxidase activity, but retained characteristic Mg2+-ATPase activity that is sensitive to vanadate, stimulated by K+, and has a pH optimum near 8.5. These data support our assumption that the upper phase of the gradient consist of the plasma membrane of S. platensis.

Original languageEnglish
Pages (from-to)737-741
Number of pages5
JournalPlant and Cell Physiology
Volume35
Issue number5
DOIs
StatePublished - 1 Jan 1994

Keywords

  • ATPase activity
  • Cyanobacteria
  • Phase partition
  • Plasma membrane
  • Spirulina platensis

Fingerprint

Dive into the research topics of 'Isolation and characterization of the plasma membrane by two-phase partitioning from the alkalophilic cyanobacterium <i>Spirulina platensis</i>'. Together they form a unique fingerprint.

Cite this