Isolation and functional analysis of peridroplet mitochondria from murine brown adipose tissue

Jennifer Ngo; Ilan Y. Benador; Alexandra J. Brownstein; Laurent Vergnes; Michaela Veliova; Michael Shum; Rebeca Acín-Pérez; Karen Reue; Orian S. Shirihai; Marc Liesa

doi:10.1016/j.xpro.2020.100243

Isolation and functional analysis of peridroplet mitochondria from murine brown adipose tissue

Jennifer Ngo, Ilan Y. Benador, Alexandra J. Brownstein, Laurent Vergnes, Michaela Veliova, Michael Shum, Rebeca Acín-Pérez, Karen Reue, Orian S. Shirihai, Marc Liesa

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Mitochondria play a central role in lipid metabolism and can bind to lipid droplets. However, the role and functional specialization of the population of peridroplet mitochondria (PDMs) remain unclear, as methods to isolate functional PDMs were not developed until recently. Here, we describe an approach to isolate intact PDMs from murine brown adipose tissue based on their adherence to lipid droplets. PDMs isolated using our approach can be used to study their specialized function by respirometry. For complete information on the use and execution of this protocol, please refer to Benador et al. (2018).

Original language	English
Article number	100243
Journal	STAR Protocols
Volume	2
Issue number	1
DOIs	https://doi.org/10.1016/j.xpro.2020.100243
State	Published - 19 Mar 2021
Externally published	Yes

Keywords

Metabolism
Molecular biology

ASJC Scopus subject areas

Neuroscience (all)
Biochemistry, Genetics and Molecular Biology (all)
Immunology and Microbiology (all)

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10.1016/j.xpro.2020.100243

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title = "Isolation and functional analysis of peridroplet mitochondria from murine brown adipose tissue",

abstract = "Mitochondria play a central role in lipid metabolism and can bind to lipid droplets. However, the role and functional specialization of the population of peridroplet mitochondria (PDMs) remain unclear, as methods to isolate functional PDMs were not developed until recently. Here, we describe an approach to isolate intact PDMs from murine brown adipose tissue based on their adherence to lipid droplets. PDMs isolated using our approach can be used to study their specialized function by respirometry. For complete information on the use and execution of this protocol, please refer to Benador et al. (2018).",

keywords = "Metabolism, Molecular biology",

author = "Jennifer Ngo and Benador, {Ilan Y.} and Brownstein, {Alexandra J.} and Laurent Vergnes and Michaela Veliova and Michael Shum and Rebeca Ac{\'i}n-P{\'e}rez and Karen Reue and Shirihai, {Orian S.} and Marc Liesa",

note = "Funding Information: M.L. is funded by UCLA :DDRC NIH P30DK41301 , 1R01AA026914-01A , and the Seed Award of DGSOM to K.R., M.L., and L.V. O.S.S. is funded by R01 DK099618-05 . We thank Linsey Stiles at the Mitochondria and Metabolism Core at UCLA. Publisher Copyright: {\textcopyright} 2020 The Author(s)",

year = "2021",

month = mar,

day = "19",

doi = "10.1016/j.xpro.2020.100243",

language = "English",

volume = "2",

journal = "STAR Protocols",

issn = "2666-1667",

publisher = "Cell Press",

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TY - JOUR

T1 - Isolation and functional analysis of peridroplet mitochondria from murine brown adipose tissue

AU - Ngo, Jennifer

AU - Benador, Ilan Y.

AU - Brownstein, Alexandra J.

AU - Vergnes, Laurent

AU - Veliova, Michaela

AU - Shum, Michael

AU - Acín-Pérez, Rebeca

AU - Reue, Karen

AU - Shirihai, Orian S.

AU - Liesa, Marc

N1 - Funding Information: M.L. is funded by UCLA :DDRC NIH P30DK41301 , 1R01AA026914-01A , and the Seed Award of DGSOM to K.R., M.L., and L.V. O.S.S. is funded by R01 DK099618-05 . We thank Linsey Stiles at the Mitochondria and Metabolism Core at UCLA. Publisher Copyright: © 2020 The Author(s)

PY - 2021/3/19

Y1 - 2021/3/19

N2 - Mitochondria play a central role in lipid metabolism and can bind to lipid droplets. However, the role and functional specialization of the population of peridroplet mitochondria (PDMs) remain unclear, as methods to isolate functional PDMs were not developed until recently. Here, we describe an approach to isolate intact PDMs from murine brown adipose tissue based on their adherence to lipid droplets. PDMs isolated using our approach can be used to study their specialized function by respirometry. For complete information on the use and execution of this protocol, please refer to Benador et al. (2018).

AB - Mitochondria play a central role in lipid metabolism and can bind to lipid droplets. However, the role and functional specialization of the population of peridroplet mitochondria (PDMs) remain unclear, as methods to isolate functional PDMs were not developed until recently. Here, we describe an approach to isolate intact PDMs from murine brown adipose tissue based on their adherence to lipid droplets. PDMs isolated using our approach can be used to study their specialized function by respirometry. For complete information on the use and execution of this protocol, please refer to Benador et al. (2018).

KW - Metabolism

KW - Molecular biology

UR - http://www.scopus.com/inward/record.url?scp=85112729480&partnerID=8YFLogxK

U2 - 10.1016/j.xpro.2020.100243

DO - 10.1016/j.xpro.2020.100243

M3 - Article

C2 - 33458705

AN - SCOPUS:85112729480

SN - 2666-1667

VL - 2

JO - STAR Protocols

JF - STAR Protocols

IS - 1

M1 - 100243

ER -

Isolation and functional analysis of peridroplet mitochondria from murine brown adipose tissue

Abstract

Keywords

ASJC Scopus subject areas

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