Isolation and molecular characterization of Leishmania infantum in urine from patients with visceral leishmaniasis in Brazil

Manoel Sebastião da Costa Lima, Andressa Cristina Lopes Hartkopf, Rosianne A. de Souza Tsujisaki, Elisa Teruya Oshiro, Julie Teresa Shapiro, Maria de Fatima Cepa Matos, Maria Elizabeth Cavalheiros Dorval

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Leishmania infantum is a protozoan that causes visceral leishmaniasis, a potentially deadly neglected tropical disease. The gold standard for diagnosis has traditionally been detection of amastigotes in bone marrow or spleen aspirates, but this is an invasive procedure that carries the risk of serious complications. Newer PCR techniques are opening new avenues and tissues for testing. Therefore, we tested if amastigotes and DNA from L. infantum could be detected in patient urine. We detected L. infantum DNA in six out of 30 urine samples from patients with visceral leishmaniasis and the promastigotes were isolated in culture from the urine of one patient. These results suggest the feasibility of using urine samples to diagnose visceral leishmaniasis, especially in acute cases or renal infection, providing a valuable tool for doctors and clinicians to use for screening and diagnosis of leishmaniasis in patients.

Original languageEnglish
Pages (from-to)248-251
Number of pages4
JournalActa Tropica
Volume178
DOIs
StatePublished - 1 Feb 2018
Externally publishedYes

Keywords

  • Diagnosis
  • Leishmania
  • PCR
  • Urine
  • Zoonosis

ASJC Scopus subject areas

  • Infectious Diseases
  • Parasitology

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