TY - JOUR
T1 - Isolation and molecular characterization of Leishmania infantum in urine from patients with visceral leishmaniasis in Brazil
AU - da Costa Lima, Manoel Sebastião
AU - Hartkopf, Andressa Cristina Lopes
AU - de Souza Tsujisaki, Rosianne A.
AU - Oshiro, Elisa Teruya
AU - Shapiro, Julie Teresa
AU - de Fatima Cepa Matos, Maria
AU - Cavalheiros Dorval, Maria Elizabeth
N1 - Publisher Copyright:
© 2017 Elsevier B.V.
PY - 2018/2/1
Y1 - 2018/2/1
N2 - Leishmania infantum is a protozoan that causes visceral leishmaniasis, a potentially deadly neglected tropical disease. The gold standard for diagnosis has traditionally been detection of amastigotes in bone marrow or spleen aspirates, but this is an invasive procedure that carries the risk of serious complications. Newer PCR techniques are opening new avenues and tissues for testing. Therefore, we tested if amastigotes and DNA from L. infantum could be detected in patient urine. We detected L. infantum DNA in six out of 30 urine samples from patients with visceral leishmaniasis and the promastigotes were isolated in culture from the urine of one patient. These results suggest the feasibility of using urine samples to diagnose visceral leishmaniasis, especially in acute cases or renal infection, providing a valuable tool for doctors and clinicians to use for screening and diagnosis of leishmaniasis in patients.
AB - Leishmania infantum is a protozoan that causes visceral leishmaniasis, a potentially deadly neglected tropical disease. The gold standard for diagnosis has traditionally been detection of amastigotes in bone marrow or spleen aspirates, but this is an invasive procedure that carries the risk of serious complications. Newer PCR techniques are opening new avenues and tissues for testing. Therefore, we tested if amastigotes and DNA from L. infantum could be detected in patient urine. We detected L. infantum DNA in six out of 30 urine samples from patients with visceral leishmaniasis and the promastigotes were isolated in culture from the urine of one patient. These results suggest the feasibility of using urine samples to diagnose visceral leishmaniasis, especially in acute cases or renal infection, providing a valuable tool for doctors and clinicians to use for screening and diagnosis of leishmaniasis in patients.
KW - Diagnosis
KW - Leishmania
KW - PCR
KW - Urine
KW - Zoonosis
UR - http://www.scopus.com/inward/record.url?scp=85037371592&partnerID=8YFLogxK
U2 - 10.1016/j.actatropica.2017.12.011
DO - 10.1016/j.actatropica.2017.12.011
M3 - Article
C2 - 29221850
AN - SCOPUS:85037371592
SN - 0001-706X
VL - 178
SP - 248
EP - 251
JO - Acta Tropica
JF - Acta Tropica
ER -