@article{c029934073454b548360310cf3f5fd18,
title = "Isolation of phaeobacter sp. From larvae of atlantic bonito (sarda sarda) in a mesocosmos unit, and its use for the rearing of european seabass larvae (dicentrarchus labrax l.)",
abstract = "The target of this study was to use indigenous probiotic bacteria in the rearing of seabass larvae. A Phaeobacter sp. strain isolated from bonito yolk-sac larvae (Sarda sarda) and identified by amplification of 16S rDNA showed in vitro inhibition against Vibrio anguillarum. This Phaeobacter sp. strain was used in the rearing of seabass larvae (Dicentrarchus labrax L.) in a large-scale trial. The survival of seabass after 60 days of rearing and the specific growth rate at the late exponential growth phase were significantly higher in the treatment receiving probiotics (p < 0.05). Microbial community richness as determined by denaturing gradient gel electrophoresis (DGGE) showed an increase in bacterial diversity with fish development. Changes associated with the administration of probiotics were observed 11 and 18 days after hatching but were not apparent after probiotic administration stopped. In a small challenge experiment, seabass larvae from probiotic treatment showed increased survival (p < 0.05) after experimental infection with a mild pathogen (Vibrio harveyi). Overall, our results showed that the use of an indigenous probiotic strain had a beneficial impact on larval rearing in industry-like conditions.",
keywords = "Antagonism, Challenge test, Fish microbiota, Larviculture, Microbiology",
author = "Pavlos Makridis and Fotini Kokou and Christos Bournakas and Nikos Papandroulakis and Elena Sarropoulou",
note = "Funding Information: This research was funded by the Commission of the European Communities through the project ?From capture based to self-sustained aquaculture and domestication in bluefin tuna, Thunnus thynnus? (GA 212797), Specific RTD program of Framework Programme 7, SELFDOTT, and by the General Secretariat for Research and Technology, Ministry of Education and Religious Affairs, Culture and Sports, Greece through the project ?Alternative preventive and therapeutic measures in Greek mariculture? which was funded by the call: Operational-Strategic Reference Framework, NSFR 2007?2013, Cooperation 2009, Action I. We thank Pantelis Katharios, Institute of Marine Biology, Biotechnology, and Aquaculture (IMBBC), Hellenic Center for Marine Research, Heraklion, Crete, for providing us the three strains of Vibrio anguillarum used in the in vitro inhibition tests, Nikos Mitrizakis for technical assistance in the rearing of seabass larvae, and Stelios Fodelianakis for running DDGE analysis. Funding Information: Funding: This research was funded by the Commission of the European Communities through the project “From capture based to self-sustained aquaculture and domestication in bluefin tuna, Thunnus thynnus” (GA 212797), Specific RTD program of Framework Programme 7, SELFDOTT, and by the General Secretariat for Research and Technology, Ministry of Education and Religious Affairs, Culture and Sports, Greece through the project “Alternative preventive and therapeutic measures in Greek mariculture” which was funded by the call: Operational-Strategic Reference Framework, NSFR 2007–2013, Cooperation 2009, Action I. Publisher Copyright: {\textcopyright} 2021 by the authors. Licensee MDPI, Basel, Switzerland.",
year = "2021",
month = jan,
day = "1",
doi = "10.3390/microorganisms9010128",
language = "English",
volume = "9",
pages = "1--13",
journal = "Microorganisms",
issn = "2076-2607",
publisher = "MDPI AG",
number = "1",
}