Isolation of stem cells using spheroids from fresh surgical specimen: An analytic mini-review

Itzhak Avital, Alexander Stojadinovic, Hongjian Wang, Ciaran Mannion, Williams C.S. Cho, Jinlian Wang, Yan Gao Man

Research output: Contribution to journalReview articlepeer-review

10 Scopus citations


It is a commonly held belief that adult stem cells represent the "seeds" for normal cellular replenishment and also for carcinogenesis. The identification and characterization of stem cells for clinical therapeutic applications, however, is extremely challenging for a number of reasons. Recently, our group and others have attempted to isolate stem cells using spheroids from fresh surgical specimens and utilize them for in vitro and in vivo studies. This mini-review summarizes the major technical steps of these methods along with the primary findings. Besides, it critically analyzes the advantages and limitations of the concept and technical approaches. Finally, this mini-review presents our thoughts on the potential future directions of stem cell isolation and cancer stem cell-related research and clinical applications.

Original languageEnglish
Pages (from-to)57-66
Number of pages10
JournalCancer Genomics and Proteomics
Issue number2
StatePublished - 1 Jan 2014
Externally publishedYes


  • Adult stem cells
  • Carcinogenesis
  • Cellular replenishment
  • Review.
  • Spheroid
  • Surgical specimens

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cancer Research


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