TY - JOUR
T1 - Isolation, structure determination, and antiaging effects of 2,3-pentanediol from endophytic fungus of Curcuma amada and docking studies
AU - Tiwari, Sudeep
AU - Singh, Sailendra
AU - Pandey, Pallavi
AU - Saikia, Shilpi K.
AU - Negi, Arvind Singh
AU - Gupta, Shailendra K.
AU - Pandey, Rakesh
AU - Banerjee, Suchitra
N1 - Funding Information:
The authors wish to express their sincere thanks to the director, CSIR-CIMAP, for providing the necessary facilities to carry out this research. Thanks are also due to Council of Scientific and Industrial Research (CSIR), New Delhi, India, for the financial support.
PY - 2014/1/1
Y1 - 2014/1/1
N2 - An endophytic fungus was isolated from the rhizomes of Curcuma amada (Zingiberaceae), which was identified as Fusarium oxysporum on the basis of its morphological and molecular characters. Chromatographic separation and spectroscopic analysis of the fungal metabolite (chloroform extract) led to the identification of one pure compound having molecular formula C5H12O2, i.e., 2,3-pentanediol (1). Activity analysis of compound 1 demonstrated improved antiaging (antioxidant, thermotolerance) properties against Caenorhabditis elegans, in comparison to a similar, commercially available molecule i.e., 1,5-pentanediol (2). The effective (lower) concentration of 1 significantly showed (28.6 %) higher survival percentage of the worms under thermal stress (37 oC) compared to its higher concentration (25.3 %), while similar trends were followed in oxidative stress where (22.2 %) higher survival percentage was recorded in comparison to untreated control. The compound 1, however, lacked potential antimicrobial activity, indicating the plausible ramification of the position of OH group in such bioactive molecules. In silico evaluation of these molecules against common as well as unique targets corroborated better antiaging potential of 1 in comparison to that of 2. The results for the first time indicated that the utilization of the endophytic fungi of C. amada could, thus, be a possible source for obtaining non-plant-based bioactive compounds having broader therapeutic applications pertaining to age-related progressions.
AB - An endophytic fungus was isolated from the rhizomes of Curcuma amada (Zingiberaceae), which was identified as Fusarium oxysporum on the basis of its morphological and molecular characters. Chromatographic separation and spectroscopic analysis of the fungal metabolite (chloroform extract) led to the identification of one pure compound having molecular formula C5H12O2, i.e., 2,3-pentanediol (1). Activity analysis of compound 1 demonstrated improved antiaging (antioxidant, thermotolerance) properties against Caenorhabditis elegans, in comparison to a similar, commercially available molecule i.e., 1,5-pentanediol (2). The effective (lower) concentration of 1 significantly showed (28.6 %) higher survival percentage of the worms under thermal stress (37 oC) compared to its higher concentration (25.3 %), while similar trends were followed in oxidative stress where (22.2 %) higher survival percentage was recorded in comparison to untreated control. The compound 1, however, lacked potential antimicrobial activity, indicating the plausible ramification of the position of OH group in such bioactive molecules. In silico evaluation of these molecules against common as well as unique targets corroborated better antiaging potential of 1 in comparison to that of 2. The results for the first time indicated that the utilization of the endophytic fungi of C. amada could, thus, be a possible source for obtaining non-plant-based bioactive compounds having broader therapeutic applications pertaining to age-related progressions.
KW - 1,5-pentanediol
KW - 2,3-pentanediol
KW - Antiaging
KW - Caenorhabditis elegans
KW - Curcuma amada
KW - Endophytic fungus
KW - Fusarium oxysporum
KW - Molecular docking
KW - Oxidative stress
UR - http://www.scopus.com/inward/record.url?scp=84905683297&partnerID=8YFLogxK
U2 - 10.1007/s00709-014-0617-0
DO - 10.1007/s00709-014-0617-0
M3 - Article
C2 - 24515569
AN - SCOPUS:84905683297
SN - 0033-183X
VL - 251
SP - 1089
EP - 1098
JO - Protoplasma
JF - Protoplasma
IS - 5
ER -