Isothermal Amplification Strategies for Detection in Microfluidic Devices

Saheli Sarkar; Pooja Sabhachandani; Tania Konry

doi:10.1016/j.tibtech.2016.09.006

Isothermal Amplification Strategies for Detection in Microfluidic Devices

Saheli Sarkar, Pooja Sabhachandani, Tania Konry

Research output: Contribution to journal › Short survey › peer-review

20 Scopus citations

Abstract

Isothermal rolling circle amplification (RCA) is used to detect nucleic and non-nucleic acid biomarkers with high sensitivity. Immuno-RCA, the specific detection of proteins via antigen–antibody recognition, has been miniaturized for microfluidic platforms to reduce reagent and sample consumption, accelerate reaction kinetics, and enhance the sensitivity and specificity of detection.

Original language	English
Pages (from-to)	186-189
Number of pages	4
Journal	Trends in Biotechnology
Volume	35
Issue number	3
DOIs	https://doi.org/10.1016/j.tibtech.2016.09.006
State	Published - 1 Mar 2017
Externally published	Yes

Keywords

droplets
immuno-rolling circle amplification
microfluidics
protein

ASJC Scopus subject areas

Biotechnology
Bioengineering

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10.1016/j.tibtech.2016.09.006

Cite this

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abstract = "Isothermal rolling circle amplification (RCA) is used to detect nucleic and non-nucleic acid biomarkers with high sensitivity. Immuno-RCA, the specific detection of proteins via antigen–antibody recognition, has been miniaturized for microfluidic platforms to reduce reagent and sample consumption, accelerate reaction kinetics, and enhance the sensitivity and specificity of detection.",

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author = "Saheli Sarkar and Pooja Sabhachandani and Tania Konry",

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AU - Sarkar, Saheli

AU - Sabhachandani, Pooja

AU - Konry, Tania

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Y1 - 2017/3/1

N2 - Isothermal rolling circle amplification (RCA) is used to detect nucleic and non-nucleic acid biomarkers with high sensitivity. Immuno-RCA, the specific detection of proteins via antigen–antibody recognition, has been miniaturized for microfluidic platforms to reduce reagent and sample consumption, accelerate reaction kinetics, and enhance the sensitivity and specificity of detection.

AB - Isothermal rolling circle amplification (RCA) is used to detect nucleic and non-nucleic acid biomarkers with high sensitivity. Immuno-RCA, the specific detection of proteins via antigen–antibody recognition, has been miniaturized for microfluidic platforms to reduce reagent and sample consumption, accelerate reaction kinetics, and enhance the sensitivity and specificity of detection.

KW - droplets

KW - immuno-rolling circle amplification

KW - microfluidics

KW - protein

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U2 - 10.1016/j.tibtech.2016.09.006

DO - 10.1016/j.tibtech.2016.09.006

M3 - Short survey

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AN - SCOPUS:85000443113

SN - 0167-7799

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EP - 189

JO - Trends in Biotechnology

JF - Trends in Biotechnology

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ER -

Isothermal Amplification Strategies for Detection in Microfluidic Devices

Abstract

Keywords

ASJC Scopus subject areas

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