Issledovanie konformatsionnykh izmeneniǐ alkogol'degidrogenazy pri vzaimodeǐstvii s nositelem silokhromom metodom EPR.

Translated title of the contribution: Study of conformational changes in alcohol dehydrogenase during its interaction with silochrome adsorbent by the EPR spectroscopy method

N. V. Kharakhonycheva, G. I. Likhtenshteǐn, E. A. Shkileva, M. D. Adamenkova

Research output: Contribution to journalArticlepeer-review

Abstract

The possible use of EPR spectroscopy (spin labelling) for the study of horse liver alcohol dehydrogenase with a silochrome adsorbent is discussed. The rotatory diffusion of nitroxyl labels chemically linked to the enzyme was studied with reference to the time of the enzyme incubation with the adsorbent and the degree of its accumulation on the adsorbent surface. The mobility of nitroxyl radicals attached to the protein globules was shown to increase with time. It was concluded that the conformation of the enzyme molecules changes during their interaction with the adsorbent.

Translated title of the contributionStudy of conformational changes in alcohol dehydrogenase during its interaction with silochrome adsorbent by the EPR spectroscopy method
Original languageRussian
Pages (from-to)970-974
Number of pages5
JournalBiokhimiya
Volume48
Issue number6
StatePublished - 1 Jun 1983

ASJC Scopus subject areas

  • Chemistry (all)

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