ITO pattern fabrication of glass platforms for electropolymerization of light sensitive polymer for its conjugation to bioreceptors on a micro-array

T. Konry, B. Hadad, Y. Shemer-Avni, S. Cosnier, R. S. Marks

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

We present herein an effective and versatile method to fabricate a micro-patterned structure of conductive polymer, poly(pyrrole-benzophenone), on Indium Tin Oxide (ITO) glass chips for the subsequent photo-immobilization of various bioreceptor, antigens. Such methodologies are based on photolithography of ITO pattern fabrication on non-conductive surfaces, glass slides, and on a photo-active electrogenerated polymer films. The photo-active polymer serves as a substrate platform for the photo-immobilization of the bioreceptor reagents used for subsequent immunoreactions. We were able to show the resolution of electropolymerization on an ITO pattern as well as immobilization of more than one bioreceptor for the simultaneous detection of several analytes. The antigen micro-arrays were tested for sensitivity, specificity, and overall practicality for the simultaneous detection of analyte anti-Cholera Toxin B, anti-Hepatitis B virus surface and core protein antibodies. In addition we used our pattern ITO-poly(pyrrole-benzophenone) micro-array for the detection of serum samples of Hepatitis B virus patients previously screened by a standard hospital detection method.

Original languageEnglish
Pages (from-to)564-571
Number of pages8
JournalTalanta
Volume75
Issue number2
DOIs
StatePublished - 15 Apr 2008

Keywords

  • Antigen micro-array
  • Cholera Toxin B
  • Hepatitis B virus
  • Indium Tin Oxide
  • Photolithography
  • Polypyrrole

ASJC Scopus subject areas

  • Analytical Chemistry

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