Keyhole limpet hemocyanin contains Gal(β1-3)-GalNAc determinants that are cross-reactive with the T antigen

Itzhak Wirguin; Ljubica Suturkova-Milosević; Chiara Briani; Norman Latov

doi:10.1007/BF01519630

Keyhole limpet hemocyanin contains Gal(β1-3)-GalNAc determinants that are cross-reactive with the T antigen

Itzhak Wirguin, Ljubica Suturkova-Milosević, Chiara Briani, Norman Latov

Research output: Contribution to journal › Article › peer-review

67 Scopus citations

Abstract

Keyhole limpet hemocyanin (KLH) is widely used as a carrier molecule to enhance immune responses to administered antigens, and for immunotherapy of bladder and renal carcinoma. In the present study we show, using lectin and antibody binding studies, that native KLH contains Gal(β1-3)GalNAc-bearing oligosaccharides, and that immunization with KLH in Lewis rats induces the production of anti-Gal(β1-3)GalNAc antibodies. This might explain the beneficial effect of KLH in bladder cancers that express crossreactive Gal(β1-3)GalNAc determinants or the T antigen.

Original language	English
Pages (from-to)	307-310
Number of pages	4
Journal	Cancer Immunology, Immunotherapy
Volume	40
Issue number	5
DOIs	https://doi.org/10.1007/BF01519630
State	Published - 1 Sep 1995
Externally published	Yes

Keywords

Immunotherapy
Keyhole limpet hemocyanin (KLH)
T antigen

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Oncology
Cancer Research

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1007/BF01519630

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title = "Keyhole limpet hemocyanin contains Gal(β1-3)-GalNAc determinants that are cross-reactive with the T antigen",

abstract = "Keyhole limpet hemocyanin (KLH) is widely used as a carrier molecule to enhance immune responses to administered antigens, and for immunotherapy of bladder and renal carcinoma. In the present study we show, using lectin and antibody binding studies, that native KLH contains Gal(β1-3)GalNAc-bearing oligosaccharides, and that immunization with KLH in Lewis rats induces the production of anti-Gal(β1-3)GalNAc antibodies. This might explain the beneficial effect of KLH in bladder cancers that express crossreactive Gal(β1-3)GalNAc determinants or the T antigen.",

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year = "1995",

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doi = "10.1007/BF01519630",

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volume = "40",

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journal = "Cancer Immunology, Immunotherapy",

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T1 - Keyhole limpet hemocyanin contains Gal(β1-3)-GalNAc determinants that are cross-reactive with the T antigen

AU - Wirguin, Itzhak

AU - Suturkova-Milosević, Ljubica

AU - Briani, Chiara

AU - Latov, Norman

PY - 1995/9/1

Y1 - 1995/9/1

N2 - Keyhole limpet hemocyanin (KLH) is widely used as a carrier molecule to enhance immune responses to administered antigens, and for immunotherapy of bladder and renal carcinoma. In the present study we show, using lectin and antibody binding studies, that native KLH contains Gal(β1-3)GalNAc-bearing oligosaccharides, and that immunization with KLH in Lewis rats induces the production of anti-Gal(β1-3)GalNAc antibodies. This might explain the beneficial effect of KLH in bladder cancers that express crossreactive Gal(β1-3)GalNAc determinants or the T antigen.

AB - Keyhole limpet hemocyanin (KLH) is widely used as a carrier molecule to enhance immune responses to administered antigens, and for immunotherapy of bladder and renal carcinoma. In the present study we show, using lectin and antibody binding studies, that native KLH contains Gal(β1-3)GalNAc-bearing oligosaccharides, and that immunization with KLH in Lewis rats induces the production of anti-Gal(β1-3)GalNAc antibodies. This might explain the beneficial effect of KLH in bladder cancers that express crossreactive Gal(β1-3)GalNAc determinants or the T antigen.

KW - Immunotherapy

KW - Keyhole limpet hemocyanin (KLH)

KW - T antigen

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DO - 10.1007/BF01519630

M3 - Article

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AN - SCOPUS:0029060612

SN - 0340-7004

VL - 40

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JO - Cancer Immunology, Immunotherapy

JF - Cancer Immunology, Immunotherapy

IS - 5

ER -

Keyhole limpet hemocyanin contains Gal(β1-3)-GalNAc determinants that are cross-reactive with the T antigen

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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