Kinetics of the release of a proton in the process of electron transfer coupled with hydrolysis of ATP in a nitrogenase molecule

L. A. Syrtsova; S. Yu Druzhinin; A. M. Uzenskaya; A. V. Khramov; A. P. Moravskii; G. I. Likhtenshtein

Kinetics of the release of a proton in the process of electron transfer coupled with hydrolysis of ATP in a nitrogenase molecule

L. A. Syrtsova, S. Yu Druzhinin, A. M. Uzenskaya, A. V. Khramov, A. P. Moravskii, G. I. Likhtenshtein

Research output: Contribution to journal › Article › peer-review

Abstract

The arrested flow method using the acid-base indicator 3,3′-dibromo-p-xylenolsulphonphthalein has been employed to investigate the kinetics of proton release during electron transfer in nitrogenase coupled with ATP hydrolysis. It is shown that all three processes occur at similar rates. It is shown that all three processes occur at similar rates. It has been established that in identical conditions (14 °C, pH 7·3) the reaction rate constant of proton release (20-23 sec^-1) matches the rate constant of electron transfer (24 ± 2 sec^-1).

Original language	English
Pages (from-to)	419-424
Number of pages	6
Journal	Biophysics (Russian Federation)
Volume	33
Issue number	3
State	Published - 1 Dec 1988
Externally published	Yes

ASJC Scopus subject areas

Biophysics

Cite this

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title = "Kinetics of the release of a proton in the process of electron transfer coupled with hydrolysis of ATP in a nitrogenase molecule",

abstract = "The arrested flow method using the acid-base indicator 3,3′-dibromo-p-xylenolsulphonphthalein has been employed to investigate the kinetics of proton release during electron transfer in nitrogenase coupled with ATP hydrolysis. It is shown that all three processes occur at similar rates. It is shown that all three processes occur at similar rates. It has been established that in identical conditions (14 °C, pH 7·3) the reaction rate constant of proton release (20-23 sec-1) matches the rate constant of electron transfer (24 ± 2 sec-1).",

author = "Syrtsova, {L. A.} and Druzhinin, {S. Yu} and Uzenskaya, {A. M.} and Khramov, {A. V.} and Moravskii, {A. P.} and Likhtenshtein, {G. I.}",

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T1 - Kinetics of the release of a proton in the process of electron transfer coupled with hydrolysis of ATP in a nitrogenase molecule

AU - Syrtsova, L. A.

AU - Druzhinin, S. Yu

AU - Uzenskaya, A. M.

AU - Khramov, A. V.

AU - Moravskii, A. P.

AU - Likhtenshtein, G. I.

PY - 1988/12/1

Y1 - 1988/12/1

N2 - The arrested flow method using the acid-base indicator 3,3′-dibromo-p-xylenolsulphonphthalein has been employed to investigate the kinetics of proton release during electron transfer in nitrogenase coupled with ATP hydrolysis. It is shown that all three processes occur at similar rates. It is shown that all three processes occur at similar rates. It has been established that in identical conditions (14 °C, pH 7·3) the reaction rate constant of proton release (20-23 sec-1) matches the rate constant of electron transfer (24 ± 2 sec-1).

AB - The arrested flow method using the acid-base indicator 3,3′-dibromo-p-xylenolsulphonphthalein has been employed to investigate the kinetics of proton release during electron transfer in nitrogenase coupled with ATP hydrolysis. It is shown that all three processes occur at similar rates. It is shown that all three processes occur at similar rates. It has been established that in identical conditions (14 °C, pH 7·3) the reaction rate constant of proton release (20-23 sec-1) matches the rate constant of electron transfer (24 ± 2 sec-1).

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SN - 0006-3509

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JO - Biophysics (Russian Federation)

JF - Biophysics (Russian Federation)

IS - 3

ER -

Kinetics of the release of a proton in the process of electron transfer coupled with hydrolysis of ATP in a nitrogenase molecule

Abstract

ASJC Scopus subject areas

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